摘要
目的观察亚慢性苯并[α]芘和铅联合染毒对小鼠行为学以及海马组织Ca2+浓度和ATP酶的影响,探讨其联合作用神经行为毒性机制。方法 80只小鼠随机分为空白对照组、溶剂对照组(植物油)、铅染毒组(54 mg/L醋酸铅)、苯并[α]芘染毒组(5 mg/kg苯并[α]芘)、铅+苯并[α]芘联合染毒组(54 mg/L醋酸铅+5 mg/kg苯并[α]芘),每组16只。以饮水行铅染毒,每星期4次腹腔注射行苯并[α]芘染毒。染毒8星期后,Morris水迷宫实验检测小鼠空间学习记忆能力,化学比色法测定海马组织钠钾ATP酶和钙镁ATP酶活性,荧光标记法测定海马组织Ca2+浓度。结果 Morris水迷宫实验结果显示:铅染毒组、苯并[α]芘染毒组、铅+苯并[α]芘联合染毒组小鼠空间学习记忆能力较空白对照组和溶剂对照组显著下降(P<0.05),且铅+苯并[α]芘联合染毒组较铅染毒组、苯并[α]芘染毒组显著下降(P<0.05)。与空白对照组和溶剂对照组比较,铅染毒组、苯并[α]芘染毒组和铅+苯并[α]芘联合染毒组海马组织Ca2+浓度显著增高(P<0.05),钠钾ATP酶和钙镁ATP酶活性显著下降(P<0.05);且与铅染毒组和苯并[α]芘染毒组比较,铅+苯并[α]芘联合染毒组小鼠海马组织Ca2+浓度显著增高(P<0.05),钠钾ATP酶和钙镁ATP酶活性显著下降(P<0.05)。结论铅和苯并[α]芘联合作用能降低小鼠的空间学习记忆能力,可能与染毒后小鼠海马组织Ca2+增多以及钠钾ATP酶和钙镁ATP酶活性下降有关。
Objective To observe the influence of sub-chronical exposure to lead and benzo （α） pyrene （B[ α] P） on mouse behaviors, calcium ion concentration and ATPase of hippocampus, and to explore joint toxical effects on the possible mechanisms of neurobehavioral toxicity. Methods Eighty mice were randomly divided into five groups （ n = 16） ： the blank control group, solvent control group （vegetable oil）, lead group （54 mg/L lead acetate）, B[ α] P group （5 mg/kg B [ α] P）, and lead ＋ B[α] P group （54 mg/L lead acetate ＋ 5 mg/kg B[ α] P）. Lead acetate dissolved in drinking water was given every day, while B[ α] P was administered intraperitoneally four times a week. After being exposed for eight weeks, the learning and memory functions of mice were measured by Morris water maze. The concentration of Ca^2＋ in hippoeampus was detected by fluorescent labeling and the colorimetrie technique was used to detect the activity of Na^＋, K^ ＋ -ATPase and Ca^2＋ , Mg^2＋ -ATP in hippocampus. Results The results of morris water maze test showed that mice given lead or/and B [ α] P exhibited a significant decrease in spatial learning and memory as compared to control groups （ P 〈 0. 05）. Lead ＋ B [ α] P group was decreased far more than lead group and B[ α] P group （P 〈 0. 05）. The levels of Na^＋, K^＋ -ATPase and Ca^2＋, Mg^2＋ -ATPase of the hippocampus in lead or/and B[ α] P group were significantly decreased （ P 〈 0.05）, while levels of Ca^2＋ were significantly increased as compared to control groups （P 〈 0.05）. The lead ＋ B[ α] P group had significantly lower activities of Na^＋, K ^＋ -ATPase and Ca^2＋, Mg^2＋-ATPase （P 〈 0. 05） and much higher concentration of Ca^2＋than those of lead group and B[α] P group （P 〈 0.05）. Conclusion The combination of lead and B[ α] P can impair the learningand memory functions of mice, and the possible mechanisms of behavioral deficits may be due to increased Ca^2＋ concentration and the decreased activities of Na ^＋ , K ^＋ -ATPase and Ca^2 ＋ , Mg^2 ＋ -ATPase in the hippoeampus.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2014年第2期134-138,共5页
Journal of Shanghai Jiao tong University:Medical Science
基金
国家自然科学基金(81041077)~~
