摘要
目的探讨18°×4扇形窥盘法正常新生儿网织红细胞计数参考范围的动态变化及其与日龄的关系。方法用18°×4扇形窥盘法对415例1～10d正常新生儿进行网织红细胞计数,并同时用国际血液学标准化委员会(ICSH)所推荐的网织红细胞计数Miller窥盘法进行比较,将两种方法所得结果按日龄与检测方法对数据进行分组,用统计学软件SPSS12.0进行处理。结果18°×4扇形窥盘法的新生儿网织红细胞计数参考范围,1d组(3.09%～6.81%)与2d组(3.00%～6.34%)、7d组(0.27%～1.15%)与8d组(0.32%～1.28%)、8d组与9d组(0.34%～1.38%)、9d组与10d组(0.35%～1.53%)之间分别进行比较,差异无统计学意义(P>0.05);2d组与3d组(1.93%～4.03%)、3d组与4d组(1.35%～3.13%)、4d组与5d组(1.26%～1.98%)、5d组与6d组(0.72%～1.45%)、6d组与7d组之间分别进行比较,差异有显著统计学意义(P<0.01);所有不同日龄组18°×4扇形窥盘法和Miller窥盘法测定的网织红细胞计数值分别进行比较,差异无统计学意义(P>0.05)。结论正常新生儿网织红细胞计数参考范围随着日龄的变化而变化,且有低值期的存在;18°×4扇形窥盘法可作为Miller窥盘法的替代方法测定新生儿网织红细胞;该参考范围的确定为网织红细胞计数在实验室操作和临床应用方面提供了依据。
Objective To explore the dynamic change of the reference range of normal newborn reticulocyte count and relationship to the day age of newborn by 18°×4 fan-shaped peep-plate method. Methods Counted of reticulocyte of 415 normal newborns from age of 1 day to 10 days with 18°×4 fan-shaped peep-plate method ,and compared the result counted with Miller peep-plate method recommended by International Committee for Standardisation in Hematology （ICSH）. Divided the values into groups by the newborn day age and different detecting method,then deal with the results with SPSS 12.0 software. Results Compared the reference range of newborn reticulocyte by the 18°×4 fan-shaped peep-plate method of 1 d age（3.09 % - 6.81%） with those of 2 d（3.00 % - 6.34 %）,7 d （0.27 % - 1.15 %） with those of 8 d（0.32 % - 1.28 %）,8 d with those of 9 d （0.34 % - 1.38%） ,9 d with those of 10 d （0.35 % - 1.53 %） ,and all the differences had no statistical significance （P 〉 0.05）. Compared the reference range of newborn reticulocyte counted by the 18°×4 fan-shaped peep-plate method of 2 d age with those of 3 d（1.93 % - 4.03 %） ,3 d with those of 4 d（1.35 % - 3.13 %） ,4 d with those of 5 d（1.26 % - 1.98 %） ,5 d with those of 6 d （0.72 % - 1.45 % ）,6 d with those of 7 d ,and the statistical significant difference were found among these groups （P 〈 0.01 ）. Furthermore,the difference results in newborn reticuloeyte values counted by the 18°×4 fan-shaped peep-plate method had no statistical significance with the results of that counted by the Miller peep-plate method （P 〉 0.05）. Conclusion There is a variation along with the reference range of newborn retieulocyte and their day age, and existing a low value period. Therefore the 18°×4 fan-shaped reticuloeyte enumerative peep-plate method can take the place of the Miller peep-plate to determine the reticulocyte value of the newborn. Thus the definite reference range of the newborn retieulocyte establishes the foundation to laboratory performing and clinical application.
出处
《生物医学工程与临床》
CAS
2008年第6期487-489,共3页
Biomedical Engineering and Clinical Medicine
