摘要
目的评价一种改良的网织红细胞染色液。方法对改良网织红细胞染色液进行显微镜镜下观察试验、染色时间试验、有效期试验、准确度试验和精密度试验。结果改良网织红细胞染色液染色后的标本在显微镜镜下具有不易褪色、沉渣少、视野清晰、背景明亮等特点,且改良网织红细胞染色液染色时间短(5 min),有效期长(30个月),准确度与煌焦油蓝盐水染色液一致(P>0.05),精密度好于煌焦油蓝盐水染色液。结论改良网织红细胞染色液是一种优秀的网织红细胞染色液,值得推广应用。
Objective To evaluate a kind of modified reticulocyte （Ret） staining solution. Methods The observation test of the modified Ret staining solution was performed under the microscope,counted the staining time, analyzed the effective life and tested its accuracy and precision. Results The modified Ret staining samples had a lot of characters, such as not easily fade, few residues, clear visual field and bright background, etc. Compared with the routine staining solution-brilliant eresyl blue, the staining time was short（5 rain） and the effective life was long（30 months）. The accuracy of the modified staining solution was consistent with that of the brilliant cresyl blue （P〉 0.05）, but the precision of the modified staining solution was better than that of the brilliant cresyl blue. Conclusion The modified Ret staining solution is an excellent staining. It is worthy to be spreader in practice.
出处
《生物医学工程与临床》
CAS
2009年第3期247-250,共4页
Biomedical Engineering and Clinical Medicine
关键词
染色液
网织红细胞
准确度
精密度
staining solution
reticulocyte
accuracy
precision
