摘要
设置了0、1、4、16、64、256mg·L-16个浓度的硝酸镧梯度溶液,运用彗星电泳测试技术,以彗星尾长、彗星率、尾距和Olive尾距来评价La(NO3)3对蚕豆根尖细胞DNA的损伤。结果表明:与对照组相比,染毒组的彗星尾长、彗星率、尾矩和Olive尾矩随浓度增加而逐渐上升。一定剂量的稀土元素镧(≥1mg·L-1)可以使蚕豆根尖细胞DNA出现损伤,在1～64mg·L-1范围内随镧浓度的增加伤害程度加大,256mg·L-1时,遗传物质损伤严重,彗星尾长、尾距和Olive尾距反而下降,说明硝酸镧具有一定的细胞毒性和遗传毒性。
Lanthanum is one of the rare chemicals which are widely used in agriculture in China because of their abilities to increase the yield and to improve quality of crops. But it also has aroused the people＇s attention to the environmental pollution by the rare-earth elements. To study the genotoxicity of lanthanum nitrate in the root tip cell of Viciafaba, six treated groups with different lanthanum nitrate content （0, 1, 4, 16, 64, 256 mg· L^-1 respectively）were designed. Comet assay was used to detect DNA damage of root tip cells of Viciafab a according to the tail length, comet rate, tail moment, olive tail moment. The results showed that the tail length, comet rate, tail moment, olive tail moment of five treated groups were increased significantly with increasing of lanthanum nitrate concentration compared with the control group. It mean that DNA of root tip cell of Viciafaba can be damaged by lanthanum nitrate. In 1-64 mg·L^-1 the damage extent was enhanced with increasing of lanthanum nitrate concentration. Genetic material was serious damaged in the concentration of 256 mg·L^-1. On the contrary, the tail length, comet rate and tail moment were descent. It suggests that lanthanum nitrate has genotoxicity and cytotoxicity to root tip cells of Viciafaba.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2008年第6期2387-2390,共4页
Journal of Agro-Environment Science
基金
国家自然科学基金(30470421)
江苏省环洪泽湖生态农业生物技术重点实验室开放基金(HZHL0811)
江苏省高校自然科学基础研究项目(08KJD180010)
关键词
彗星实验
遗传毒性
硝酸镧
蚕豆根尖细胞
comet assay
genotoxicity
lanthanum nitrate
root tip cells of Viciafaba
