摘要
目的采用HPLC同时测定滇产紫丹参中丹参素钠、原儿茶醛、咖啡酸、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA。方法采用Hypersil ODS色谱柱(4.6 mm×250 mm,5μm),乙腈-0.1%磷酸溶液为流动相,梯度洗脱,体积流量:1.0 mL/min;检测波长:280 nm。结果本方法各主要成分色谱峰之间有良好的分离度,6个成分的浓度和各自峰面积之间有着良好的线性关系,加样回收率的RSD均小于3.0%。结论该方法简便、快速,结果准确,可用于紫丹参的质量控制。
AIM To develop an HPLC method for determing sodium danshensu,protocate chuicaldehyde,caffeic acid,cryptotanshinone,tanshinoneⅠ and tanshinone ⅡA simultaneously in Salvia yunnanensis Roots.METHODS The column was Hypersil ODS（4.6 mm × 250 mm,5μm）.The mobile phase consisted of acetonitrile（A） -water（containing 0.1% phosphoric acid）（B） in the gradient mode.The flow-rate was 1.0 mL/min.The detecting wave length was set at 280 nm.RESULTS The relationship between the concentration and the peak areas of the six compounds was all linear.The RSD of recovery were all less than 3.0%.CONCLUSION The method is simple,rapid and accurate and reproducible,and could be used for the quality control of Salvia yunnanensis C.H.Wright.
出处
《中成药》
CAS
CSCD
北大核心
2011年第4期648-650,共3页
Chinese Traditional Patent Medicine
基金
昆明理工大学分析测试研究中心主任基金(2008-07)
关键词
紫丹参
HPLC
丹参素钠
原儿茶醛
咖啡酸
隐丹参酮
丹参酮Ⅰ
丹参酮ⅡA
Salvia yunnanensis Roots
HPLC
sodium danshensu
protocate chuicaldehyde
caffeic acid
cryptotanshinone
tanshinoneⅠ
tanshinone ⅡA
