摘要
应用彗星试验对地塞米松诱导的小鼠胸腺凋亡细胞与重铬酸钾诱导的普通DNA链断裂损伤细胞进行了比较研究。结果发现 :在彗星形态上 ,凋亡细胞具有明显的特征 ;在相同电泳条件下 ,凋亡细胞的彗星出现时间早 ;在剂量 反应关系上 ,凋亡细胞表现为凋亡指数增加 ,而DNA链断裂损伤细胞为彗星尾长增加 ;在修复的时效关系上 ,凋亡继续发展 ,普通DNA链断裂损伤有明显修复。研究表明 ,彗星试验不仅能敏感地检测DNA链断裂 ,而且可以了解DNA链断裂损伤的性质 ,对于研究肿瘤的发生、发展和转归具有重要意义。
Apoptosis of immature thymocyte of mice induced by dexamethason and DNA strand broken cells induced by K 2Cr 2O 7 were tested by comet assay. The results showed that the morphology of apoptotic cells assayed by comet was special. Under the same condition, the time of apoptotic cells running on electrophoresis was shorter than DNA broken cells. The length of comet tail of DNA strand broken cells increased in a dose response relation with the increase of K 2Cr 2O 7, whereas, the apoptotic index increased with the dosage of dexamethason. While DNA damage was repairing, apoptosis continued to process. The DNA strand broken cells could be obviously seen while DNA damage was repairing. It was suggested that comet assay could be used to detect DNA strand break sensitively, but also could distinguish different grades of DNA damage, which is helpful for studies on the etiology, progressing and outcome of cancer.
出处
《卫生研究》
CAS
CSCD
北大核心
2001年第3期149-151,共3页
Journal of Hygiene Research
基金
国家自然科学基金资助项目!(No .39670 62 5)
