摘要
以Ｐｂ－Ａｃｅｔ和５－ＨＴ静注同时ＬＰＳ灌胃方法形成肠源性内毒素血症动物模型。利用荧光素异硫氰酸盐—内毒素（ＦＩＴＣ－ＬＰＳ）的荧光特性，将其按１／１０的比例混入于口灌的ＬＰＳ中。结果证实，造模３小时后，肝、肺、肾、脑均有较强的荧光发射，说明已有肠道ＦＩＴＣ－ＬＰＳ移位现象发生。本方法具有简单性、可行性、先进性等特点，不失为一种较佳的定性观察ＬＰＳ移位的实验方法。
Entertoxemia model of mice was made by intravenous injection of Pb Acet and 5 HT with LPS by oral simutaneously.According to the fluorescent character of FITC LPS the model was made by LPS mixed with 10 percent of FITC LPS by oral.Result showed that there were stronger fluorescent emission in liver,lung,kidney and brain 3 hours after the model was made,proving the translocation of FITC LPS from gut.Fluorescent parameters used were Ex495.3nm and Em516.7nm.There was also low fluorescent intensity in normal mice tissues,but it was heterogenous substance from FITC LPS.Optimal parameters were Ex453.8nm and Em517.9nm.
出处
《中国中西医结合外科杂志》
CAS
1997年第1期39-42,共4页
Chinese Journal of Surgery of Integrated Traditional and Western Medicine
关键词
荧光素
异硫氰酸盐
内毒素
内毒素移位
动物模型
fluorescein isothiocyanate lipopolysaccharide(FITC LPS),translocation of LPS,mice
