摘要
目的探讨血清和肝组织TGF-β1水平与慢性乙肝肝纤维化程度的关系，为肝纤维化诊断提供依据。方法以肝活检病理诊断区分131例慢性HBV感染者纤维化程度（S0～S4），用ELISA法检测血清TGF-β1水平，免疫组化法检测肝组织TGF-β1表达并半定量，分析血清TGF-β1和肝组织TGF-β1表达与肝纤维化程度的关系。结果血清和肝组织TGF-β1均与肝纤维化程度具有非常显著性正相关（r分别是0．74和0．89，P〈0．01）。血清TGF-β1各组间比较差异有统计学意义（P〈0．01）。组间分割比较，S0和S1分别与S4比较差异均有统计学意义（P〈0．005）；各组与S0组比较差异均有统计学意义（P〈0．005）；S1组和S3组之间比较有统计学意义（P〈0．005）。肝组织TGF-β1表达在S3和S4组之间比较差异无统计学意义（P〉0．05），其余组间比较差异均有统计学意义（P〈0．01）。血清TGF-β1和肝组织TGF-β1表达具有非常显著性意义相关（r=0．61，P〈0．01）。结论血清TGF-β1和肝组织TGF-β1水平与慢乙肝肝纤维化程度相关，血清TGF-β1有希望成为临床判断轻度或重度肝纤维化的无创伤性诊断指标。
Objective To detect the level of Serum and liver tissue TGF-β1 in patients with chronic hepatitis B, to study their relation to liver fibrosis and gain the evidence for diagnosis of liver fibrosis. Methods The liver fibrosis grades（S0-S4） of 131 cases with chronic HBV infection were diagnosed after liver biopsy. Serum TGF-β1 was detected by enzyme-linked immunosorbent assay, and the semiquantitative analysis was applied after detecting the expression of TGF-β1 in liver tissue with immunohistochemistry. Their relations to liver fibrosis were analyzed. Results Serum and tissue level of TGF-β1 increased significantly with the development of fibrosis, and the same result was obtained between themselves（ P 〈 O. 01 ）. There was very significant difference for Serum level of TGF-β1 among the groups with different fibrosis grades （P 〈 0.01 ）. Serum levels of TGF-β1 were decreased significantly comparing the Group SO or S1 to S4（ P 〈 0.005）. There were significant difference for Serum level of TGF-β1 among SO and the others（ P 〈 0.005） . And there was significant difference between S1 and S3（ P 〈 0.005） .The expression level of TGF-β1 in liver tissue has no significant difference between group S5 and S4（ P 〉 0.05）. However, the differences were significantly among the others comparisons（ P 〈 0.01 ）. Conclusion There is close relation between the level of TGF-β1 and the different liver fibrosis grades due to chronic hepatitis B. The serum level of TGF-β1 is a potential noninvasive maker for diagnosis of liver fibrosis.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2008年第5期354-357,共4页
Chinese Journal of Experimental and Clinical Virology
基金
浙江省自然基金资助项目（Y205410）
浙江省教育厅高校科研计划项目（20050857）
关键词
转化生长因子-Β
肝硬化
肝炎病毒
乙型
肝炎/诊断
Transforming growth factor beta
Liver cirrhosis
Hepatitis B virus
Hepatitis/diagnosis
