摘要
本文研究了培养基中硝态氮与铵态氮的比例及氮源总量的改变对胀果甘草(Glycyrrhiza inflata Batal.)悬浮培养细胞的生长量和细胞内黄酮类化合物含量的影响。结果表明,当MS培养基中其他成分不变,NO3-/NH4+摩尔浓度之比为50∶10,氮源的总量为80mmol·L-1,此时获得最大的细胞生长量15.02g·L-1;以铵盐为唯一氮源,总量为40mmol·L-1,此条件下细胞内黄酮类成分含量最高,达16.74mg.g-1。在此基础上进行了两步悬浮培养方法的研究,使甘草黄酮产量达到每升培养基212.00mg,为对照的3.64倍。
The effects of nitrate-nitrogen to ammonium-nitrogen ratio and total amount of nitrogen on the cell growth and flavonoids accumulation by suspension cell culture of Glycyrrhiza inflata Batal were investigated.The maximum biomass of 15.02g·L^-1 was achieved when initial nitrogen concentration was 80 mmol·L^-1 with NO-3/NH＋4 molar ratio of 50∶10;the maximum flavonoids concentration of 16.74mg·g^-1 was achieved when ammonium as the sole N source and initial nitrogen concentration was 40 mmol·L^-1.A two-stage flask culture system was established based on the effects of nitrogen and the production of flavonoids reached to 212.00mg·L^-1 which was 3.64 times of the maximum production of single-stage process culture.
出处
《海峡药学》
2008年第10期136-139,共4页
Strait Pharmaceutical Journal
关键词
胀果甘草
氮源
黄酮类成分
两步培养
Glycyrrhiza inflata Batal
Nitrogen
Flavonoids
Two-stage process
