摘要
目的探究蒽醌类抗肿瘤抗生素与DNA的相互作用以及研究的方法。方法采用光谱法,包括吸收光谱、荧光光谱、电化学法等考察蒽醌类抗肿瘤抗生素与DNA的相互作用后引起的光谱行为的改变。结果在紫外、可见光的测定中发现嵌插结合通常引起减色效应,使最大吸收波长向长波方向移动;在(牛血清白蛋白)BSA浓度一定的条件下,随着盐酸表柔比星浓度的增加,BSA的内源荧光强度有规律的降低(发射峰的峰位λem=344nm和峰形不变);多柔比星的最大激发波长λex=478 nm,最大发射波长λem=596 nm,pH=3.0时激发光谱和发射光谱的荧光强度达到最大值。结论多柔比星与DNA的相互作用最明显,现在临床上使用最广泛。
OBJECTIVE To study the interaction of anthracene quinone type anti-tumor antibiotics and DNA as well as the experiment method. METHODS Spectrophotometry was developed including absorption spectrum, fluorescence spectrum, electrochemical method and so on. RESULTS The union of inlay and insertion observed in ultraviolet and visible spectrophotometry usually caused hypochromic effect and red shift. Under the certain concentrations of bovin serum albumin （BSA）, the endogene fluorescence intensity of BSA orderly reduced with the increase in concentration of doxorubicin （adriamycin） hydrochloride （kern 344 and the peak shape were invariable）; Xex and kern at the biggest wave length of doxorubicin were 478 and 596 nm. The fluorescence intensity was maximal of the excitation and emission spectrum when pH was 3.0. CONCLUSIONS The interaction of doxorubicin and DNA is the strongest according to the experiment and is the most widely used at present in clinics.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2008年第10期1368-1370,共3页
Chinese Journal of Nosocomiology
