摘要
目的:研究三黄泻心汤水提取液舒血管作用的可能机制。方法:记录苯肾上腺素(PE)和KCl预收缩的离体大鼠主动脉环张力变化,观察三黄泻心汤水提取液舒血管作用及不同工具药对舒血管作用的影响。结果:三黄泻心汤水提取液(0.1,0.3,0.5mg/ml)对PE(1.0μmol.L-1)和KCl(50mmol.L-1)预收缩的大鼠主动脉环均有非内皮依赖的、浓度依赖性的舒张作用。对PE(1.0μmol.L-1)预收缩的去内皮血管环,三黄泻心汤水提取液(0.5mg/ml)呈舒张作用,分别用一氧化氮合酶抑制剂左旋硝基精氨酸甲酯(L-NAME)(0.1mmol.L-1)和鸟苷酸环化酶抑制剂亚甲蓝(MB)(10μmol.L-1)预处理无明显影响。在无钙营养液(含EGTA)环境下,三黄泻心汤预处理对苯肾上腺素(PE)收缩有明显抑制作用。结论:三黄泻心汤有浓度依赖性的血管舒张作用,此作用即不依赖血管内皮,又和内皮源性的舒张因子NO无关,可能与抑制血管平滑肌细胞内质网储存钙的释放有关。
Abstract Objective： To research the vasodilative acts on thoracic aorta of rats and the possible mecha.nism of Sanhuangxiexin Decoction （SD）. Methods： Isometric tensions of the aortic ring, precontracted by phenylephrine （ PE ） and potassium chloride （KCI） respectively, were recorded in vitro when the doses of 0. 1, 0. 3, 0. 5 mg/ml of SD were cumulatively given to aortic ring. Results ： SD could relax the aortic ring precontracted by PE or KCI. The relaxant effects were＇endothelium-independent, but dose-dependent at the same time. But if pretreated with L-NAME of iNOS inhibitor and methylen blue （MB） of guanylate cyclase inhibitor, the aortic ring did not show obvious relaxation responses after treated by SD. In the absence of extraceUular Ca^2＋ , pre-incubation of the aortic rings with SD reduced significantly the contraction induced by phenylephrine （PE）. Conclusion： SD has certain concentration-dependent vasolidative effects by means of inhibition of release of Ca^2＋ from intracellular stores. The relaxant action of SD includes inhibition of Ca^2＋ , influx and release of Ca^2＋ from intracellular stores.
出处
《四川中医》
北大核心
2008年第10期11-13,共3页
Journal of Sichuan of Traditional Chinese Medicine
基金
吉林省延边大学博士科研启动基金
关键词
三黄泻心汤
主动脉
胸
血管舒张
Sanhuangxiexin Decoction （SD）
thoracic aorta
vasodilation
