摘要
目的研究苦碟子的内皮依赖性血管舒张作用,并探讨其可能机制。方法采用大鼠离体主动脉环灌流模型,观察累积浓度苦碟子对基础状态、去氧肾上腺素(PE)和氯化钾(KCl)预收缩的内皮完整血管环和去内皮血管环的舒张作用。结果苦碟子水提取液(苦碟子注射液,相当于含苦碟子原材料0.01～10g.L-1)对基础状态的内皮完整血管环和去内皮血管环的张力无影响。对PE预收缩的内皮完整血管环,苦碟子在累积浓度0.03～10g.L-1呈浓度依赖性舒张作用,分别用一氧化氮合酶抑制剂左旋硝基精氨酸甲酯(0.1mmol.L-1)和鸟苷酸环化酶抑制剂亚甲蓝(10μmol.L-1)预处理,此作用被抑制,用环氧合酶抑制剂吲哚美辛(10μmol.L-1)预处理亦被抑制。对KCl预收缩的内皮完整血管环和对PE或KCl预收缩的去内皮血管环,苦碟子在累积浓度0.01～10g.L-1无明显舒缩作用。结论苦碟子(0.03～10g.L-1)对主动脉具有内皮依赖性舒张作用。该作用可能是通过血管内皮细胞一氧化氮-鸟苷酸环化酶途径和血管内皮细胞环氧合酶途径介导的。
AIM To investigate the endotheliumdependent vasodilation effect of the water extract from Ixeris sonchifolia （Bge.） Hance （WEISH） in rat thoracic aorta and its possible mechanism. METHODS WEISH 0.01 - 10 g· L^-1（ injection, corresponding to raw material） was cumulatively added into organ bath. Isometric tension of endothelium-intact or endothelium-denuded thoracic aortic rings in basal tension, preconstricted by phenylephrine （PE） and potassium chloride （ KCI）, respectively, was recorded. RESULTS Cumulative administration of WEISH 0.01 - 10 g· L^-1 did not affect the vasomotion of aortic rings with endothelium or without endothelium in basal tension. Exposure of intact endothelium rings preconstricted by PE to WEISH at the concentrations of 0.03 - 10 g· L^-1 induced a significant concentration-dependent relaxation, which was inhibited by preincubation with Nω-nitro-L-arginine methyl ester, methylene blue and indometacin, respectively. Exposure of intact endothelium rings preconstricted by KCl and endothelium-denuded rings preconstricted by PE or KCl to WEISH at the concentrations of 0.03 - 10 g· L^-1 did not affect the vasomotion. CONCLUSION WEISH 0.03 - 10 g· L^-1 has endothelium-dependent relaxation effect on aortic rings. This effect may be mediated by the nitric oxide-guanylyl cyclase pathway and cyclooxygenase pathway.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2007年第3期202-206,共5页
Chinese Journal of Pharmacology and Toxicology
关键词
苦碟子
主动脉
胸
血管舒张
内皮
Ixeris sonchifolia （ Bge. ） Hance
aorta, thoracic
vasodilation
endothelium
