摘要
利用共振散射技术研究了金纳米微粒与溶菌酶的相互作用。金纳米微粒可以通过静电引力及疏水作用与溶菌酶结合,使金纳米微粒粒径变大,从而导致纳米金瑞利共振散射光谱显著增强,并且在一定范围内光散射强度的增加量与溶菌酶浓度成正比。考查了作用时间、溶液酸度、共存离子及有机溶剂等条件的影响。将纳米金作为测定溶菌酶的探针,在最佳反应条件下,对溶菌酶的检出限为0.08μg/mL。将此方法用于蛋清中溶菌酶的测定,检测结果与文献报道方法一致,结果令人满意。
The interaction of gold nanoparticles （ NCs ） and lysozyme was studied with resonance rayleigh scattering spectra. Gold NCs can be linked with lysozyme by the virtue of electrostatic and hydrophobic interaction, which arouse the aggregating of bigger diameters of gold NCs and strongly enhancing of their resonance rayleigh scattering intensity. The enhancing RRS intensity was in proportion to the concentration of lysozyme within a range of 0. 4 - 9.6 μg/mL. The effects of reaction time, pH of solution, coexisted ions and organic regents were studied in this work. Under optimum conditions, the detection limit of lysozyme is 0.08 μg/mL with gold NCs as a probe. This method was successfully used to the detection of lysozyme in egg white, which was consistent with other reports.
出处
《激光生物学报》
CAS
CSCD
2008年第5期679-683,共5页
Acta Laser Biology Sinica
基金
辽宁省教育厅科学研究基金项目(2006B112)
辽东学院科研基金资助项目(2006-Z08)
关键词
纳米金
溶菌酶
瑞利共振散射光谱
gold NCs
lysozyme
resonance rayleigh scattering spectra
