摘要
目的 建立简便、快捷的大量制备质粒的实验方法。方法 以文献报道方法为依据 ,将传统的质粒小量抽提的实验方法与大量抽提的实验方法有机结合 ,以琼脂糖凝胶电泳的方法证明了使用改进后方法的可行性。结果 使用该法可以一次大量地制备质粒DNA ( 690± 2 19) μg ,提取效力为 ( 1 4± 0 4) μg/ml菌液 ,并可直接用于酶切。结论 改进后的实验方法更加简便、快捷、高效 。
Objective To establish a more convenient technique for large-scale preparation of plasmid DNA from Escherichia coli than before.Methods Based on the literature′s method,the author reasonably combined the conventional small-scale extraction technique with a large-scale one,and then checked the feasibility of the newly improved technique by agarose gel electrophoresis.Results Its production output was (690±219) μg plasmid DNA at one time,extraction rate was (1.4±0.4) μg per milliliter culture medium.The product can be directly digested by restriction enzyme.Conclusion The improved technique is more convenient than before,which provides a practical method for large-scale preparation of plasmid DNA.
出处
《中国药物与临床》
CAS
2003年第2期103-105,共3页
Chinese Remedies & Clinics
基金
山西省归国留学人员基金资助项目 ( 2 0 0 0 41)
