摘要
以国际上公认的化感水稻PI312777和非化感水稻Lemont(均引自美国)为材料,采用水培方法研究了不同水稻品种响应低磷(0.5mg/L,以P计)胁迫的分子生理特性.结果表明,低磷胁迫促进PI312777根的生长,对株高和干重具有微弱的抑制作用.而Lemont相应形态指标均受到较大的抑制,与PI312777相比,均达到极显著差异.低磷胁迫对PI312777相应生理生化指标的影响均低于Lemont,且达极显著水平.此外,低磷胁迫下,PI312777植株氮(N)、磷(P)、钾(K)含量均不同程度高于Lemont,具体表现为两种水稻植株N含量差异不显著,而P、K含量差异达极显著水平.N、P、K吸收利用相关的12个关键酶的基因差异表达进一步证明了前述结论.研究显示,低磷胁迫下,PI312777比Lemont具有更强的适应性能力.
Allelopathic rice PI312777 and non-allelopathic rice Lemont were employed as test materials to explore physiobiochemical and molecular response of different allelopathic potential rice accessions under phosphorus deficiency in hydroponics. The two rice accessions were cultured in Hoagland solution with 0.5 mg （P） L^-1 as phosphorus deficiency （P-） and 6 mg （P） L^-1 as normal phosphorus supply （P＋）. Their morphological indices and physiobiochemical characteristics were investigated at the two phosphorus supplying levels. Furthermore, the differential expression of 12 genes encoding the key enzymes associated with absorption and utilization of nitrogen, phosphorus and potassium was detected in rice by Real Time Fluorescent Quantitative PCR （FQ-PCR） to evaluate the expression changes in the two rice accessions with lower phosphorus supply. The results indicated that with lower phosphorus supply, the root length of PI312777 was enhanced, but the plant height and dry weight were slightly reduced. The same tendency was also observed in the case of Lemont. However, the effect of low phosphorus stress on the physiobiochemical characteristics of non-allelopathic rice Lemont was significantly higher than that of allelopathic rice PI312777. Further study showed that the content of nutrient elements in PI312777 was higher than that in Lemont. The contents of nitrogen in the two rice accessions were not significant, while the contents of phosphorus and potassium in PI312777 were significantly higher than those in Lemont under low phosphorus treatment. The differential expression of the 12 genes involved in nutrient absorption and utilization was defected under low phosphorus treatment and the result was consistent with that as mentioned above. These findings suggested that allelopathic rice PI312777 had better adaptability and endurance to nutrient deficiency than non-allelopathic rice Lemont.
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2008年第5期593-598,共6页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金(Nos.30471028,30671220)
福建省自然科学基金重大项目(No.2002F012)~~
