摘要
利用农杆菌介导的叶盘法 ,把HAL1基因转入番茄 ,Southern杂交检测得到转基因植株。耐盐实验表明 ,T1代转基因番茄在 15 0mmol/L的NaCl胁迫下仍有 4 3%的发芽率 ,2 0 0mmol/L的NaCl胁迫下发芽率为 6 % ,而对照种子在 10 0和 15 0mmol/L的NaCl胁迫下发芽率分别为 11.0 %和 0。转基因番茄的电解质相对外渗率小于对照 ,而根冠比和叶绿素含量大于对照 ,转HAL1基因显著提高了番茄的耐盐性。盐胁迫下Na+ 、K + 的累积状况表明 ,转基因番茄根、茎、叶的K+ /Na + 均有所提高 ,根系的SK/Na 增大 ,茎、叶的RSK/Na和RLK/Na减小 ,说明根系对K + /Na+ 离子的选择吸收和运输能力加强。不但选择吸收K + /Na+ ,而且表现出整株水平上的有利于耐盐的K + /Na+
HAL1 , a gene isolated from yeast ( Saccharomyces cerevisiae ) and encoding cation transport protein, was transferred by Agrobacterium mediation into tomato ( Lycopersicon esculentum Mill.) with leaf disk method, and transgenic plants were obtained after Southern hybridization. The results of salt tolerance showed that 43% seeds of transgenic tomato in T 1 generation germinated when exposed to 150 mmol/L NaCl, and 6% seeds did to NaCl 200 mmol/L (Table 1). By the contrary, tomato of wild type (control) had only 11% and 0 seeds germinated respectively on media containing 100 and 150 mmol/L NaCl. Electrolytic leakage of the transgenic plants was lower than that of the control while ratio of root/shoot and chlorophyll content of transgenic plants higher than that of control (Table 2), demonstrating that HAL1 transgene had significantly raised the level of salt tolerance of tomato plants. Under salt stress, the transgenic plants had higher ratio of K +/Na + in root, shoot and leaf, larger S K/Na in root, and smaller RS K/Na and RL K/Na in shoot and leaf (Table 3). It is demonstrated that roots of transgenic plants has stronger ability to selectively absorb K + or Na + and to transport them. The feature of selective absorption of K +/Na + was correlated with advantageous compertmentation of K +/Na + in individual plant.
出处
《植物生理与分子生物学学报》
CAS
CSCD
2003年第5期409-414,共6页
Journal Of Plant Physiology and Molecular Biology
关键词
HAL1基因
番茄
耐盐
离子吸收
分子生理
HAL1 gene
tomato
salt tolerance
ion absorption
molecular physiology
