摘要
目的：建立同时测定六味地黄丸中的马钱苷和丹皮酚的含量的方法。方法：采用高效液相色谱法，Agilent ZorbaxSBC，8色谱柱（4．6mm×250mm，5μm），流动相为乙腈-水线性梯度洗脱，柱温30℃，流速1．0mL·min^-1，检测波长236nm（马钱苷），274nm（丹皮酚）；使用matlab编程进行谱图融合。结果：马钱苷与丹皮酚的线性范围分别为0．0362～1．09（r=0．9998）；0．0450～1．35μg（r=0．9998）；平均回收率分别为97．3％（RSD1．4％）和103．0％（RSD1．9％）。结论：该方法为六味地黄丸的质控提供了更简便、合理、可靠的质控方法。
Objective： To establish a high performance liquid chromatographic（HPLC） amalgamated to double UV waves method for simultaneous determination of loganin and paeonol in Liuwei Dihuang pills. Method： A HPLC method was developed. The separation was carried out on a Agilent Zorbax SB C18 column （5 μm, 4. 6 mm ×250 mm）. The mobile phase cohsisted of water （A） and acetonitfile （B） with linear linear gradient elution [0-8 rain, （B） from 1% to 12% ; 8-21 min, B keep 12% ; 21-40 min, （B） from 12% to 90% ; 40-50 rain, B keep 90% for 10 mini. The detection was Photodiode Array with the detection wavelengths were at 236 nm and 274 nm. The column temperature being 30℃ and the flow rate was 1.0 mL ·min^-1. Extracting the chromatergraph from 274 nm and 236 nm, we amalgamated the two chromatographs by matlab programmed. Result： The calibration curves of loganin and paeonol were linear in the ranges of 0. 036 2-1.09 μg （r =0. 999 8） and 0. 045 0-1.35μg （r =0. 999 8）, respectively. The average recoveries of loganin and paeonol were 97. 3% （RSD 1. 4 % ） and 103. 0% （RSD 1.9% ）, respectively. Three different batches of Linwei Dihuang pills were determined with this method. Conclusion： This is a more convenient, reasonable and credible quality control method for the traditional Chinese medicine.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第19期2182-2184,共3页
China Journal of Chinese Materia Medica
基金
天津市科技发展计划项目(05SYSYGX25000)
关键词
六味地黄丸
高效液相色谱法
马钱苷
丹皮酚
Liuwei Dihuang pills
high performance liquid chromatography
loganin
paeonol
