摘要
[目的]为中华红叶杨种苗的快速繁殖提供依据。[方法]以红叶杨茎段为外植体,经Vc液振荡处理3 h后进行常规消毒处理,然后分别接种于附加不同含量活性炭的培养基上,不经Vc液处理为对照,研究Vc液处理和在培养基中加入活性炭及二者综合应用对红叶杨外植体褐变的影响。[结果]Vc液处理在外植体培养初期对褐变有一定的抑制作用,随时间延长其抑制作用减弱;当培养基中含0.3%的活性炭时,2周后可观察到绿色小芽点,8周后芽长达2 cm以上;对照组无明显变化;Vc液和活性炭综合应用对褐变的抑制效果最好,外植体长出的芽达0.9 cm长。[结论]用Vc液处理红叶杨外植体,同时在培养基中加入0.3%的活性炭,对外植体褐变有较好的抑制效果。
[ Objective ] The study was to provide a basis for the rapid propagation of the seedlings of Hongye poplar. [ Method] With the stem of Hongye poplar as explant, they were treated by Vc solution vibrating for 3 h before the routine disinfection, then were inoculated in the medium adding the activated carbon with different content and that without Vc solution treatment was taken as CK. The effects of treatment of Vc solution and adding activated carbon into medium and their comprehensive application on explant browning of Hongye poplar were studied. [ Result ] The treatment with Vc solution had some inhibitive effects on browning in the initial stage of the explant culture, and the inhibitive effects were weakened with the time delay. The small green buds could be observed after 2 weeks and the bud length reached over 2 cm after 8 weeks when 0. 3% activated carbon was added in the medium, and that in CK group had no obvious change. The inhibitive effect on browning was the best when Vc solution and activated carbon were comprehensively applied, and the bud of the explant was up to 0.9 cm in length. [ Conclusion] The inhibitive effects on exptant browning was good when the explant of Hongye poplar was treated by Vc solution and 0.3% activated carbon was added into the medium at the same time.
出处
《安徽农业科学》
CAS
北大核心
2008年第26期11232-11233,共2页
Journal of Anhui Agricultural Sciences
关键词
外植体
Vc液
活性炭
褐变
Explant
Vc solution
Activated carbon
Browning
