摘要
以萘为唯一碳源和能源从南冲绳海槽深海沉积物中分离得到一株能降解萘的海洋噜低温细菌Nah-1,测定了该菌的最适生长条件及生长曲线。16S rRNA基因(16S rDNA)序列同源性分析表明该菌属于解环菌属(Cycloclasticus)。PCR扩增萘降解基因得到目标片段,比对结果表明,相似度最高的基因phnA1来自Cy- cloclasticus sp.A5,为99%,该基因编码的蛋白是萘双加氧酶大亚基。
A PAHs-degrading psyehrophilic bacterium,with naphthalene as sole carbon and energy source, was isolated from deep-sea sediment of Southern Okinawa Trough. The growth curve of this bacterium was determined and the optimum growth conditions of this bacterium were studied. 16S rDNA sequences analy- sis indicated that it belongs to the genus CyclocIasticus. A fragment of the degrading dioxygenase gene was obtained through PCR from Nah-1. And sequence alignment revealed that it was similar to the phnA1 gene of Cycloclasticus sp. AS, which encoded a putative naphthalene dioxygenase large subunit.
出处
《海洋科学》
CAS
CSCD
北大核心
2008年第10期30-34,共5页
Marine Sciences
基金
中国科学院知识创新工程重要方向项目(KZC)(2-YW-211-03)
关键词
萘
多环芳烃
生物降解
双加氧酶
深海嗜低温细菌
naphthalene
polycyclic aromatic hydrocarbon
biodegradation
dioxygenase
psychrophilic deep-sea bacterium
