摘要
研究了海洋微生物SS6在无机盐培养基中对多环芳烃菲的降解和解毒作用和发光细菌对菲及其降解产物毒性的反应.结果表明:SS6菌能利用菲作为唯一的碳源和能源生长;培养液中有酸性代谢产物产生,并且形成积累;发光细菌毒性测试技术可以有效地检测菲及其微生物降解中间产物的相对毒性;母体菲的毒性远大于其水杨酸代谢途径的中间产物水杨酸和儿茶酚及邻苯二甲酸代谢途径的中间产物邻苯二甲酸毒性,并且邻苯二甲酸代谢途径的解毒效果优于水杨酸代谢途径;菲降解过程中培养液萃取物的毒性逐渐降低,但上清液的毒性先出现下降后又呈增大趋势;含菲培养液的毒性变化是培养液中存在的中间产物和其它组成成分及pH值变化的综合结果.
The ability of mixed cultures SS6 isolated from coastal sediments to degrade and detoxify poly-cyclic aromatic hydrocarbon phenanthrene was studied in a basal liquid medium, and the Microtox test was used to assay the toxicity of phenanthrene and its metabolites . It has been found that SS6 is capable of using phenanthrene as sole carbon and energy sources, and acid by-products of incomplete degradation might be produced and accumulated in medium during the incubation period. The biotoxicity of phenanthrene and its metabolites were successfully assayed by Microtox test. Phenanthrenen was more toxic than its metabolites, such as salicylate, catechol and o-phthalate. And the detoxification through salicylate route was superior to that of through o - phthalate route. Microtox test demonstrated a decrease in the toxicity of dichloromethane culture extracts from the culture containing phenanthrenen, and a decrease followed with an increase in the toxicity of culture supernatants over the incubation period. The occurrence of water - soluble phenanthrenen transformation products and change of the component of the medium and pH value must be responsible for the toxicity of the culture supernate together.
出处
《集美大学学报（自然科学版）》
CAS
北大核心
2004年第3期193-199,共7页
Journal of Jimei University：Natural Science
基金
国家自然科学基金资助项目(30370276)福建省教育厅科研基金资助项目(KB0316)
