摘要
目的研究Fas相关死亡结构域(FADD)蛋白在膀胱尿路上皮癌(BUC)组织的表达,并探讨其与肿瘤生物学行为之间的关系。方法采用免疫组化SP法检测68例BUC及13例正常膀胱黏膜组织FADD蛋白表达。原位杂交法检测其中34例BUC组织FADD mRNA表达水平。结果正常膀胱黏膜FADD蛋白呈阳性表达。FADD的阳性率在不同组织学分级、不同临床分期的BUC组织中差异均有极显著性(χ2=15.38、11.34,P<0.01);复发组与未复发组FADD的阳性率分别为36.84%和53.33%,差异无显著意义(2χ=1.85,P>0.05)。BUC组织中FADD mRNA阳性率为44.12%,FADD蛋白阳性率为47.06%,两者差异无统计学意义(χ2=0.06,P>0.05)。结论FADD表达异常在BUC的发展中可能起着重要作用,FADD阳性表达与BUC病理分级、浸润关系密切,但与BUC复发无关。
Objective To investigate the Fas-associated death domain （FADD） protein and its biological behavior in bladder urothelial carcinoma （BUC）. Methods The expressions of FADD in 68 BUC and 13 normal bladder tissue were detected by streptavidin-peroxidase immunohistochemical technique. Of which, 34 BUC were studied by in-situ hybridization for FADD mRNA. Results The various exprssions of FADD were as follows： it positively expressed in normal bladder mucosa; the differences of positive expression were significant among different histological grading and clinical staging （X^2= 15.38,11.34;P〈0.01）; its expression in recurrent group and non-recurrent group was 36.84% and 53.33%, respectively, the difference was insignificant （X^2= 1.85 ,P〉0.05）. In BUC, the positive FADD mRNA was 44.12% and FADD protein was 47.06%, with no statistical difference was noted between them （X^2 = 0.06, P〉0.05）. Conclusion The abnormal expression of FADD may play an important role in the development of BUC. The positive expression of FADD in the cancer is closely related to the pathological grading and in- vasion, but not related to its recurrence.
出处
《青岛大学医学院学报》
CAS
2008年第5期436-437,440,共3页
Acta Academiae Medicinae Qingdao Universitatis
关键词
FAS相关死亡结构域蛋白
膀胱肿瘤
免疫组织化学
原位杂交
Fas-associated death domain protein
Bladder neoplasmas
Immunohistochemistry
In situ hybridization
