摘要
目的:研究凋亡促进基因PDCD5在多发性骨髓瘤(MM)中的表达,分析其与抑凋亡基因BCL-2的相关性,初步探讨PDCD5在MM发病机制中的作用。方法:采用免疫组织化学染色、流式细胞检测术和逆转录-聚合酶链反应(RT-PCR)方法测定MM患者组和对照组骨髓单个核细胞PDCD5和BCL-2蛋白和mRNA的表达,同时对两者进行相关性分析。结果:免疫组织化学结果显示,PDCD5蛋白阳性细胞率MM组为(34.75±6.49)%,对照组为(52.98±5.84)%;PDCD5染色强度指数(SII)MM组为281.16±75.33,对照组为462.84±39.77;BCL-2蛋白阳性细胞率MM组(29.97±5.57)%,对照组(5.56±1.95)%;BCL-2蛋白SII在MM组为224.94±57.72,对照组为27.84±9.75;以上指标两组比较差异均有统计学意义(P<0.05)。流式细胞技术检测,骨髓浆细胞中PDCD5蛋白阳性率MM组(78.11±21.63)%,对照组(89.46±9.98)%;蛋白平均荧光强度MM组为61.73±11.04,对照组为353.04±123.26;以上两组比较差异均有统计学意义(P<0.05)。RT-PCR检测,PDCD5mRNA的相对表达水平MM组为0.33±0.07,对照组为0.53±0.05;BCL-2mRNA的相对表达水平MM组为0.33±0.08,对照组为0.12±0.02;以上两组比较差异均有统计学意义(P<0.05)。PDCD5与BCL-2表达相关性研究结果,PDCD5与BCL-2蛋白阳性细胞率表达呈负相关(r=-0.86,P<0.05);PDCD5与BCL-2mRNA相对表达水平呈负相关(r=-0.90,P<0.05)。结论:MM患者骨髓单个核细胞PDCD5蛋白和mRNA表达下调;BCL-2蛋白和mRNA表达上调;PDCD5与BCL-2蛋白阳性细胞率和mRNA表达均呈负相关。
Objective To determine the expression of apoptosis related gene PDCD5 in multiple myeloma （ MM ） , and to analyze the relation between PDCD5 and BCL-2. Methods The expressions of PDCD5 and BCL-2 protein and mRNA were determined by immunohistochemical staining method, flow cytometry （FCM） and reverse transcription polymerase chain reaction （RT-PCR）method in bone marrow mononuclear cells. We also analyzed the relation between PDCD5 and BCL-2. Results Immunohistochemical staining showed that PDCD5 protein positive cell percentage, staining intensity index （SII） of PDCD5 protein, BCL-2 protein positive cell percentage, and SII of BCL-2 protein were （34.75 ±6.49）%, （281. 16±75.33）, （29.97 ±5.57）%, and （224.94± 57.72） in the MM group and （52.98±5.84）%, （462.84±39.77）, （5.56±1.95） %, and （27.84±9.75） in the control group （all P 〈0.05）. Results of FCM showed that PDCD5 protein positive percentage and mean fluorescence intensity of PDCD5 were （78.11 ± 21.63 ）% and （61.73 ±11.04） in the MM group and （89.46 ±9.98）% and （353.04 ± 123.26） in the control group （ all P 〈 0. 05 ） . RT-PCR showed that relative expression of PDCD5 and BCL-2 mRNA were （0.33±0.07） and （0. 33 ±0.08） in the MM group and （0. 53 ±0.05） and （0.12 ± 0.02 ） in the control group （ all P 〈 0.05 ）. The positive cell percentage of PDCD5 and BCL-2 protein was negative correlation （ r = - 0. 86, P 〈 0. 05 ） ; the expression of PDCD5 and BCL-2 mRNA was the same status （ r = - 0. 90 ,P 〈 0.05 ） . Conclusion The expressions of PD- CD5 protein and mRNA in MM patients are down-regulated, but the expressions of BCL-2 protein and mRNA are up-regulated. The mRNA and protein expression of PDCD5 and BCL-2 has negative correlation.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2008年第9期814-820,共7页
Journal of Central South University ：Medical Science
基金
湖南省自然科学基金(06JJ50152)~~
