摘要
为探讨抑凋亡基因Ｂｃｌ－２与子宫内膜癌发生发展及预后的关系，用ＡＢＣ免疫组化法对４０例正常子宫内膜和６０例子宫内膜癌Ｂｃｌ－２蛋白表达状况进行检测。结果发现Ｂｃｌ－２表达在正常子宫内膜中呈周期性变化，其表达由增生早期开始逐渐增强，到增生晚期表达最强，进入分泌期则减弱乃至消失。子宫内膜癌中Ｂｃｌ－２表达水平远远低于增生期内膜Ｂｃｌ－２表达水平，且表达于预后较好的腺棘癌、乳头状腺癌、单纯腺癌组织，而浆液性腺癌和鳞癌组织中则无表达；分化程度低、期别晚者，Ｂｃｌ－２表达弱（Ｐ＜０．０１）；在５例伴有内膜增生过长的内膜癌中，增生过长内膜的表达明显强于癌组织和增生期内膜的表达。由此推测Ｂｃｌ－２高表达可能在子宫内膜增生过长的发生中起着重要作用，而内膜癌中Ｂｃｌ－２表达明显减弱，其作用仍待深入研究。Ｂｃｌ－２的高表达可提示预后较好。
To make an initial inquiry into the correlation of development and prognosis with the expression of Bcl 2 protein in endometrial carcinoma, we detected the Bcl 2 protein expression in 40 normal and 60 malignant samples of the endometrium by means of an immunohistochemical technique. We observed that Bcl 2 expression varied with menstrual cycle, becoming stronger in proliferative phase and then declining in secretory phase. Bcl 2 expression in endometrial carcinoma was much weaker than that in normal endometrium, and Bcl 2 was particulary expressed in adenoacanthoma, papillary adenocarcinoma and simple adenocarcinoma. The higher grade and stage were, the stronger Bcl 2 expression was ( P<0.01 ). In 5 cases of endometrial carcinoma with hyperplastic endometrium, Bcl 2 expression of carcinoma was obviously weaker than that of hyperplastic endometrium. So we conclude that Bcl 2 overexpression may contribute to the formation of hyperplastic endometrium, that obvious reduction of Bcl 2 expression in endometrial carcinoma may promote apoptosis of cells, and that Bcl 2 over expression may be associated with good prognosis.
出处
《华西医科大学学报》
CSCD
1999年第1期92-95,103,共5页
Journal of West China University of Medical Sciences
关键词
子宫内膜癌
细胞凋亡
BCL-2基因
Endometrial carcinoma Apoptosis B cell lymphoma leukemia 2 gene Immunohistochemistry
