摘要
目的利用蛋白质组学方法，识别剥苔差异表达的蛋白质，为进一步探讨病理舌苔产生机制奠定基础。方法运用固相pH梯度双向凝胶电泳技术分离正常组和剥苔组舌苔组织的总蛋白质、银染显色、凝胶扫描入计算机，ImageMaster2DPlatinum软件分析比较电泳图谱。应用图象分析软件比较分析，识别差异表达的蛋白质；应用质谱仪（massspectrometry）得到相应的肽质指纹图谱（脚tidemassfingerprint，PMF），搜索数据库鉴定差异蛋白质。结果建立正常舌苔和剥苔双向凝胶电泳图谱：对四组舌苔组织的蛋白质组进行分离分析，发现凝胶中可检测的蛋白质斑点数分别为1082±105个和1143±140个：较正常组8个蛋白质点表达上调、7个表达下调，鉴定出9种差异表达蛋白质。结论建立了分辨率和重复性均较好的双向电泳银染图谱：可识别和鉴定出一些与剥苔相关的差异表达蛋白质。
Objective To investigate the microbiostatic mechanism of denuded tongue coating, Proteomic methods were used to find differential expression proteins in denuded tongue. Methods The total protein of normal or denuded tongue coating was separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis （2-DE）. The differential expression protein was analyzed by using ImageMaster 2D analysis software. Image analysis software was used for comparative analysis to find differential expression proteins; Mass spectrometry （mass spectrometry） was applied to get the corresponding peptide fingerprint （peptide mass finger print. PMF）. Search the database to identify differences in protein. Results Establish two-dimensional gel electrophoresis for normal and denuded tongue; Segregation analysis on four groups of proteome of tongue coating showed that gel could be detected in the protein spots at 1082±105 and 1143±140 respectively; Compared with normal group, there are eight points of protein expression being regulated upward and seven points down. Altogether 9 differences in protein expression were identified. Conclusion The sound resolution and repeatability Two-dimensional electrophoresis silver staining pattern were established; Some differences in protein expression related to denuded tongue were identified.
出处
《国际中医中药杂志》
2008年第5期323-325,366,共4页
International Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目（项目编号：30640071）
关键词
舌苔
蛋白质组学
双向电泳
蛋白质差异表达谱
方法学
Tongue coating
Proteomics
Two-dimensional gel electrophoresis
Differences in protein expression profile
Methodology
