摘要
聚丙烯酰胺凝胶电泳目前已经广泛应用在SSR标记、SNP标记以及遗传图谱的构建等过程中,但是一直以来凝胶银染方法由于染色时间长、染色步骤繁琐,使得对于开展大批量的实验研究极为不利。文章通过对常规方法的改良,建立了一个银染步骤只需要10min,整个染胶过程只需约20min的快速、高效的银染方法。
Polyacrylamide gel electrophoresis is widely applied in the SSR, SNP markers analysis and the construction of genetic map. It is disadvantage to use conventional silver-stained methods in experiment due to taking a very long time and having fussy steps. In this paper, a silver-stained method of DNA in PAGE gel was improved. The improved method is rapid banding which only takes about 20 min, and the high definition picture was obtained.
出处
《遗传》
CAS
CSCD
北大核心
2008年第10期1379-1382,共4页
Hereditas（Beijing)
基金
国家“十一五”科技支撑计划(编号:2006BAD01A1205)
国家科技基础条件平台专项(编号:2006DKA30470-002)
中国水产科学研究院水产种质资源与养殖技术重点开放实验室开放课题(编号:2006A005)资助~~
关键词
银染方法
DNA
聚丙烯酰胺凝胶
silver-stained method
DNA
polyacrylamide gel electrophoresis
