摘要
本文介绍了应用聚丙烯酰胺凝胶电泳检测DNA的改进方法。与以往方法相比,本方法从配制凝胶储备液,无需封口的水平灌胶,适当地提高电泳的电压梯度,采用改良银染法检测,省却凝胶固定,银染法DNA的纯化方法,及溴化乙锭染色法的操作等一系列改进措施,使聚丙烯酰胺凝胶电泳检测DNA的操作得到简化,更便捷,并减小了对人与环境的毒害作用。
Some improvements to the routine PAGE method for detecting DNA,was introduced,including preparation of stored gel solution.pouring gel horizontally without sealing the edges,running the-gel under higher voltage gradient, rapid staining and detection.and tactics of recovering silver stained DNA. This method is proved to be more simple and faster than previous ones and also reproducible.
出处
《细胞生物学杂志》
CSCD
1999年第4期201-203,共3页
Chinese Journal of Cell Biology
