摘要
以发现的雄性不育大鼠的正常表型同胞鼠为种鼠,通过全同胞近亲交配方式,采用选优法成功培育出自发雄性不育大鼠近交系MIJ。同系异体皮肤移植验证其遗传纯合度,观察其表型特征,并通过遗传测交试验测定雄性不育性状的遗传方式。结果表明,MIJ种群符合近交系标准;雄性不育大鼠呈睾丸下降延迟、睾丸及附睾发育迟缓、精子数量少及精子生成障碍;近交系内其他个体表型正常。不育性状呈隐性遗传方式,由常染色体上单一隐性基因控制。该雄性不育大鼠可作为人类男性不育研究的动物模型。
The normal sibling rats of the infertile male were selected for brother-sister breeding. The stocks of every filial generation whose male siblings were infertile were inbred with their brothers or sisters by optimum seeking method. After 20 generations,a rat inbred strain of spontaneous male infertility was established, and was named MIJ. The genetic homozygosity was analyzed by skin grafting. The phenotypes of MIJ were observed, and the mode of infertility inheritance was studied by test cross experiments. The results demonstrated that MIJ rats had reached the norm of inbred strain, and the infertile male rats showed the phenotypes of delayed testicular descent, testicular and epididymis hypoevolutism, oligozoospermia and hypospermatogenesis. The phenotypes of the other rats in the inbred strain were normal. The infertility mutation was inherited in a recessive mode, and was controlled by a monogenic recessive gene in euchromosome. The infertile male rats could serve as a useful model for the study of human infertility.
出处
《动物学杂志》
CAS
CSCD
北大核心
2008年第5期37-44,共8页
Chinese Journal of Zoology
基金
河北省科技攻关计划项目(No.07275503)
\河北省科技条件建设项目(No.2008090008)
关键词
雄性不育
近交系
大鼠
遗传特征
小睾丸
Male infertility
Inbred strain
Rat
Genetic characteristic
Small testis
