摘要
目的探讨重症急性胰腺炎(SAP)大鼠胰腺组织诱导型-氧化氮合成酶(iNOS)mRNA的表达及其与肠壁通透性的关系,并观察丹参对其的影响。方法大鼠随机分为3组:(1)SAP组(n=15);(2)SAP丹参注射液治疗组(T组,n=15);假手术对照组(C组,n=15)。原位杂交法检查各组胰腺iNOS mRNA表达,血中一氧化氮(NO),淀粉酶(AML),内毒素(LPS)和血中与肠内125I-清蛋白累积指数,以及胰腺和回肠病理检查。结果SAP组胰腺iNOSmRNA呈强阳性表达,血中NO,AML和LPS含量及125I-清蛋白累积指数和腹水量均显著高于C组(P<0.01)。与SAP组比较,T组胰腺及回肠病理损害明显减轻,胰腺iNOSmRNA表达强度较弱,血中NO,AML,LPS及腹水量均显著下降(P<0.01),125I-清蛋白累积指数亦降低。结论SAP肠壁通透性增加的原因之一可能与SAP时胰腺iNOSmRNA的过度表达产生过多NO有关。丹参通过下调胰腺iNOSmRNA的表达,对降低SAP的肠壁通透性有一定预防治疗作用。
Objective To investigate the relationship between inducible nitric oxide synthase （iNOS） mRNA expression of pancreatic tissues and intestinal permeability, and the effect of salviae mihiorrhizae on the change in gut permeability in rats with severe acute pancreatitis （SAP）. Methods All rats were randomly divided into three groups ： Sham operation （ group C, n = 15 ） , SAP group （ group SAP, n = 15 ） , and salviae mihiorrhizae therapeutic group （group T, n = 15 ）. At 24h After operation, the iNOS mRINIA expressions of pancreas were determined by in situ hybridization （ISH） , and blood amylase （AMY） , nitric oxide （NO） and lipopolysaccharide （LPS） were examined. The histopathologie changes of pancreas and ileum were observed. Intestinal permeability was measured by means of albumin clearance（ AC ）of ^125I-labeled rat serum albumin. Results The iNOS mRNA expressions of pancreas in group SAP were markedly increased in comparison with group T （ P 〈 0.01 ） , while there was no iNOS mRNA expression of pancreas in group C. The blood AML, NO, LPS and ascites and intestinal permeability in group SAP were much higher than those of group C（P〈0.01） ,and also higher than those of group T （P 〈0.01） except AC. The pathological changes of pancreas and ileum in group SAP were more severe than in group T （ P 〈 0. 01 ） , however pathology of pancreas and ileum was normal in group C. Conclusions Excess NO produced by pancreatic iNOS mRNA overexpression may be a factor related to intestinal permeability in SAP. Salvia miltiorrhiza can down-regulate the iNOS mRNA expression of pancreas and improve the intestinal permeability in SAP.
出处
《中国普通外科杂志》
CAS
CSCD
2008年第9期848-851,共4页
China Journal of General Surgery
基金
贵州省优秀科技教育人才省长基金资助项目(黔科教办2001-06)
关键词
胰腺炎
急性坏死性/病理学
一氧化氮合成酶
原位杂交
肠壁通透性
丹参
Pancreatitis, Acute Necrotizing/pathol
Inducible Nitris Oxide Synthase,
Situ Hybridization
Intestinal Permeability
Salvia Mihiorrhiza
