摘要
目的探讨大黄素在急性胰腺炎（AP）时对胰腺诱生型一氧化氮合酶（iNOS）和血清瘦素表达，以及对肠壁通透性的影响及作用机制。方法60只成年SD大鼠随机分为AP组（n=20），假手术组（n=20），和大黄素组（n=20）。检测血清淀粉酶活性、血清瘦素含量、胰腺组织中iNOS及NO含量、肠黏膜通透性（以血液与肠内125I-清蛋白累积指数表示），并对胰腺和回肠进行病理学检查。结果AP组和大黄素组血清淀粉酶活性、瘦素含量、胰腺组织内的NO和iNOS水平及125I-清蛋白累积指数均显著高于假手术组（P〈0．05）；与AP组比较，大黄素组血清淀粉酶活性、胰腺组织内的NO、iNOS水平及125I-清蛋白累积指数均显著下降（P〈0．05），血中瘦素含量明显升高（P〈0．05），同时胰腺及回肠病理损害明显减轻。结论AP肠壁通透性增加的原因之一可能与AP时胰腺iNOS的过度表达产生过多NO有关，大黄素可降低胰腺iNOS的表达，该作用可能与升高血清瘦素水平有关，大黄素对降低AP时肠壁通透性的增加有一定预防和治疗作用。
Objective To investigate the effects of emodin on inducible nitric oxide synthase （iNOS） expression of pancreatic tissue, serum leptin and intestinal permeability in rats with acute pancreatitis（AP） and the relative mechanism. Methods Sixty adult SD rats were randomly divided into three groups： sham operation group, AP group, and emodin treatment group, with 20 in each group. The expressions of iNOS and NO in pancreatic tissue were measured. Blood amylase（AMY） and serum content of leptin were detected. Intestinal permeability was measured by albumin clearance（AC） of 125I-labeled rat serum albumin. The histopathologic changes of pancreas and ileum were observed. Results The blood AMY, plasma content of leptin, cumulative 125I-labeled serum albumin index and the levels of iNOS and NO in pancreatic tissue were significantly higher in AP group and emodin treatment group than in sham operation group（P 〈 0.05）. Compared with AP group, in emodin treatment group, the levels of iNOS and NO in pancreatic tissue, the blood AMY and cumulative 125I-labeled serum albumin index were significantly decreased（P 〈 0.05）; the serum content of leptin was increased; the pathological changes of pancreas and ileum were significantly alleviated（P 〈 0.05）. Conclusion The increase of intestinal permeability in rats with AP may be induced by overexpression of NO via action of iNOS. Emodin is effective in the increase of the serum leptin and the decrease of iNOS. Emodin has certain preventive and therapeutic effects on bowel wall permeability decrease in AP rats.
出处
《中华老年多器官疾病杂志》
2012年第2期138-141,共4页
Chinese Journal of Multiple Organ Diseases in the Elderly
关键词
急性胰腺炎
瘦素
一氧化氮合酶
肠壁通透性
大黄素
acute panereatitis
leptin
nitric oxide synthase
intestinal permeability
emodin
