摘要
采用V(甲醇)∶V(甲酸)=19∶1溶液提取杨梅中的花色苷,并用阳离子交换树脂初步纯化得到杨梅花色苷提取物,然后以水-正丁醇-甲基叔丁基醚(TBME)-乙腈-三氟乙酸(TFA)(体积比5∶2∶1.5∶1∶0.001)为溶剂系统,用高速逆流色谱对杨梅花色苷进行分离。再由C18柱色谱纯化后,得到2种主要的花色苷单体Ⅰ和Ⅱ。经ESI-MS和NMR鉴定为矢车菊色素-3-β-吡喃型葡萄糖苷(Ⅰ)和飞燕草色素-3-葡萄糖苷(Ⅱ)。另外,文中对3个杨梅品种的花色苷组成进行了分析,结果表明,矢车菊色素-3-β-吡喃型葡萄糖苷是杨梅花色苷的主要组成成分,占花色苷总量的90%以上。
The anthocyanins in waxberry were extracted by the mixture solvent composed of methanolformic acid （9 ： 1, v/v） and pre-purified by cation exchange resin column chromatography. The waxberry was then separated by countercurrent chromatography with a ,solvent system composed of water-n-butanoltert butyl methyl ester-acetonitrile- trifuoroacetic acid （5 ： 2 ： 1.5 ： 1： 0. 001, v/v）, and purified with C18 column chromatography to yield cyanidin-3-O-β- D -glucopyranoside （Ⅰ） and delphinidin-3-O-β- D -Glucopyr- anoside （Ⅱ）. The chemical structure was elucidated by ESI-MS, ^1H-NMR and ^13C-NMR. The composition analysis of anthocyanins in three waxberries showed that cyanidin-3-O-β-D-glucopyranoside was the main anthocyanin monomer, more than 90% of the total anthocyanins.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2008年第8期48-51,55,共5页
Food and Fermentation Industries
基金
教育部重点科技项目(204062)
关键词
杨梅
花色苷
结构鉴定
组成分析
waxberry, anthocyanin, structure elicidation, composition analysis.
