摘要
以老黑谷米为对象,在单因素试验的基础上,利用二次通用旋转试验设计,对老黑谷米色素的提取工艺进行了优化,并对色素的体外抗氧化活性进行了评价。结果表明:老黑谷米色素的最佳提取溶剂为乙醇,在282 nm和342 nm处有最大吸收波长,初步定性为黄酮醇类物质;该色素的最佳提取条件为提取温度30℃、液料比20∶1(m L/g)、提取时间40 min;该色素具有较好的抗氧化活性,DPPH自由基清除能力为(3 512.56±26.28)μg阿魏酸/g,铁离子还原/抗氧化能力为(44.04±6.60)mmol Fe^(2+)/L,还原能力为(203.47±23.75)μg抗坏血酸/g,H_2O_2清除活性为(3 676.60±114.32)μg阿魏酸/g,ABTS^+·清除能力为(2 778.70±262.90)μg Trolox/g。
The extraction process for pigments from the grains of ＂Laoheigu＂ rice, a red-grained rice variety grown in Pantiange town, Diqing prefecture, Yunnan province was optimized by quadratic general rotary design based on single factor experiments and the antioxidant activities in vitro of the extracted pigments were investigated. The results showed that the best extraction solvent for the pigments was determined as alcohol and the maximum absorption peaks appeared at 282 and 342 nm, suggesting that they were flavonol compounds. The optimum extraction conditions were as follows： extraction temperature, 30 ℃; liquid to material ratio, 20：1（m L/g）; and extraction time, 40 min. The pigments had potent antioxidant activities; DPPH radical scavenging activity was（3 512.56 ± 26.28） μg ferulic acid equivalents/g; ferric reducing antioxidant power（FRAP） was（44.04 ± 6.60） mmol Fe^（2＋） equivalents/L; reducing power（RP） was（203.47 ± 23.75） μg ascorbic acid equivalents/g; hydrogen peroxide scavenging activity was（3 676.60 ± 114.32） μg ferulic acid equivalents/g, and 2,2＇-azinobis（3-ethylbenzothiazoline-6-sulfonate）（ABTS） radical scavenging activity was（2 778.70 ± 262.90） μg Trolox equivalents/g.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第2期19-24,共6页
Food Science
基金
国家自然科学基金面上项目(31360378
31560428)
云南省自然科学基金项目(2013FB042)
云南省高校食品加工与安全控制重点实验室项目(201401)
国家大学生创新创业训练计划项目(201510676001)
关键词
老黑谷米
色素
二次通用旋转
提取
抗氧化活性
＂Laoheigu＂ rice
pigments
quadratic general rotary design
extraction
antioxidant activity
