摘要
目的建立同步测定银黄颗粒中绿原酸和黄芩苷的HPLC方法。方法色谱柱为HYPERSIL C18（4．6mm×150mm，5μm），以甲醇-0.2％磷酸水溶液为流动相，梯度洗脱。流速为0．8mL·min^-1，检测波长为324nm，柱温25％。结果绿原酸的线性范围为0．23-1．14μg（r=0．9999），平均回收率为100．12％，RSD为0．84％；黄芩苷的线性范围为0．33-1．64μg·L^-1（r=0．9999），平均回收率为102．4％，RSD为1．47％。结论该方法简单、快速、准确，可为银黄制剂的质量控制提供实验依据。
Objective To develop an method for simultaneous determination of chlorogenic acid and baicalin in Yinhuang granules. Methods The chromatographic analysis were carried out on a HYPERSIL Clscohmn （4.6mm×150mm,5μm） with methanol-0.2% phosphoric acid as mobile phase and 0.8 mL·min^-1 flow rate in gradient mode. Results The linear ranges of chlomgenic acid and baicalin were from 0.23μg to 1.14μg （ r = 0.9999） and from 0.33μg to 1.64μg （ r = 0.9999） , the mean recoveries of chlomgenic acid and baicalin were 100. 12% with RSD of 0.84% and 102.4% with RSD of 1.47% respectively. Conclusion The method is simple, fast and accurate. It can be used to control for the quality of Yinhuang granules.
出处
《现代中药研究与实践》
CAS
2008年第4期34-35,共2页
Research and Practice on Chinese Medicines
关键词
HPLC
银黄制剂
绿原酸
黄芩苷
HPLC
Yinhuang granules
chlorogenic acid
baicalin
