摘要
目的观察蒺藜果皂苷对大鼠脑缺血后线粒体膜电位和Bcl-2mRNA、Bax mRNA表达的影响,探讨蒺藜果皂苷对大鼠脑缺血的保护作用。方法60只Wistar大鼠随机分成5组:空白对照组、模型对照组、阳性药物舒血宁组、蒺藜果皂苷低剂量组和蒺藜果皂苷高剂量组。线栓法阻断一侧大脑中动脉,缺血24h制成脑缺血模型。流式细胞仪检测线粒体膜电位,实时荧光定量PCR检测Bcl-2、Bax mRNA表达。结果模型组的线粒体膜电位和Bcl-2mRNA表达水平显著低于空白对照组(P<0.05),而阳性药物组和蒺藜果皂苷高、低剂量组的线粒体膜电位和Bcl-2mRNA表达水平明显高于模型组(P<0.01);与模型组相比,阳性药物组、蒺藜果皂苷高、低剂量组和空白对照组的Bax mRNA表达水平则明显下降,统计学上差异具有显著性(P<0.05)。结论蒺藜果皂苷能够保持线粒体膜电位,增加Bcl-2mRNA的表达而降低Bax mRNA的表达水平。
Objective To observe the effects of saponins of tribulus terrestris （STT） on mitochondrial membrane potentials and Bcl-2, Bax mRNA expression in rats after cerebral ischemia and to explore the protection roles. Methods A total of 60 male Wistar rats were randomly divided into 5 groups： normal control group, model control group, positive drug group, low dose of STT group and high dose of STT group. The model of focal cerebral ischemia was established by occluding middle cerebral artery for 24 h. The mitochondria membrane potential was detected by flow cytometry and the expression of Bcl-2 mRNA, and Bax mRNA was determined by fluorescence real-time PCR. Results Mitochondrial membrane potentials and the expression of Bcl-2 mRNA from model control group were significantly lower than the control group （P 〈 0.05）, but Mitochondrial membrane potentials and the expression of Bcl-2 mRNA from positive drug group, low dose group and high dose group were significantly higher than model control （P 〈 0.01 ）; Compared with positive drug group, normal group, low dose group and high dose group, the expression of Bcl-2 mRNA was significantly different with model control （P 〈 0.05）. Conclusions The mitochondria membrane potential is kept and the expression of Bax mRNA inhibited but improved in Bcl-2 mRNA by STT.
出处
《中华疾病控制杂志》
CAS
2008年第4期339-342,共4页
Chinese Journal of Disease Control & Prevention
基金
国家自然科学基金(30371739)
关键词
蒺藜
脑缺血
线粒体
Tribulus terrestris
Brain ischemia
Mitochondrial
