摘要
目的:探讨蒺藜皂苷(简称STT)对体外培养的肾癌细胞(786-O)的影响及其抑癌机制。方法:应用MTT法、Wright染色及AO荧光染色法和流式细胞术(FCM)及结合免疫荧光分别测定STT对细胞的生长抑制作用,细胞凋亡的形态变化,细胞周期及Bcl-2蛋白表达的影响。结果:MTT法显示STT可对786-O产生明显的细胞毒作用;Wright染色及吖啶橙荧光染色法发现STT可诱导786-O在体外产生凋亡;STT可使786-O的S期细胞比例增多;STT能够降低786-O细胞的抗凋亡蛋白Bcl-2及细胞周期蛋白D1的表达。结论:STT对786-O细胞有明显的体外生长抑制作用,并通过影响细胞周期及下调Bcl-2蛋白表达而诱导细胞凋亡。
Objective： To investigate the effect of saponins from Tribulus terrestris （STT） on the renal carcinoma ce11（786.O） in vitro, and inhibitory mechanisms. Method： Effects of STT on the cytotoxicity, morphological changes of apoptosis, cell cycle and expression of Bcl-2 protein in the 786-O were tested respectively by MTT method, Wright and acridine orange stain assay, as well as flow cytometry（FCM）. Resuit： After the 786-O was treated by STT, it was shown that： 1） A significant cytotoxic effect was observed by MTT assay; 2） Apoptosis-induced was viewed by Wright and acridine orange stain assay; 3） The distribution of 786-O on S phase was increased： 4） The expression of Bcl-2 protein and cyclin DI was decreased, conclusion： STF can significantly inhibit the growth of 786-O in vitro, partially, by apoptosis.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2005年第16期1271-1274,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金资助项目(30370158)
关键词
蒺藜皂苷
癌细胞(786-O)
细胞凋亡
saponins from Tribulus terrestris （STT）
renal carcinoma cell（786-O）
apoptosis
