摘要
传染性支气管炎病毒（ＩＢＶ）分离株ＱＤ经ＲＴ－ＰＣＲ扩增出约１．７ｋｂＳ１糖蛋白基因ｃＤＮＡ，修饰后插入ｐＵＣ１８ＳｍａｌⅠ／ＥｃｏＲＩ位点构成质粒ｐＵＣＱＤＳ１。对克隆的Ｓ１基因５′端高变区序列测定显示，起始密码上游６０碱基和下游３８０碱基中与参考株Ｍ４１Ｓ１基因序列仅有１个碱基的差异，表明ＱＤ为一类Ｍ４１分离株。
The 1.7 kb S1 gene cDNA was amplified by reverse transcriptionpolymerase chain reaction and then inserted into pUC18 Smal Ⅰ/Eco RI clone site to construct the plasmid pUC QDS1. The sequence of 5′end hypervariable region of cloned S1 gene showed that there is only one different nucleotide (nt) between QD isolate and M41 strain within 60 nt upstream and 380 downstream of start codon. The results indicated that the QD may be a M41like infectious bronchitis virus isolate.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1997年第6期535-538,共4页
Chinese Journal of Veterinary Science
基金
国家攀登计划B类项目
