摘要
目的观察Rg1、Rb1对糖尿病肾病大鼠肾组织血管内皮生长因子(VEGF)蛋白表达的影响。方法75只大鼠随机分为正常组与造模组,造模组采用空腹一次性腹腔注射链脲佐菌素复制糖尿病模型后随机分为模型组、厄贝沙坦组、Rg1大剂量组、Rg1小剂量组、Rb1组,给药12周;12周末处死大鼠,取肾后固定包埋切片,采用HE染色、Mallory染色观察肾组织病理改变,并应用免疫组化检测肾组织VEGF蛋白的表达。结果Rg1、Rb1能够降低糖尿病大鼠的蛋白尿,增加体重,降低Scr和BUN,改善肾功能,调节TG,改善脂代谢紊乱,并且能够降低糖尿病大鼠肾小管上皮细胞、肾间质细胞内的VEGF在细胞浆内的表达。结论Rg1、Rb1可抑制VEGF蛋白的过度表达,进而减缓糖尿病肾病的病程进展。
Objective： To study the expression of vascular endothelial growth factor（VEGF） in the renal tubules of STZ induced diabetic nephropathy rats. Methods： 75 SD rats were randomly divided into two groups： normal control and diabetic nephropathy. Diabetes was induced by intraperitoneal injection of STZ. And the diabetic rats were randomly divided into 4 groups： model group, irbesartan treatment group, Rg1 low and high dose treatment group, Rb1 group. All the rats were sacrificed at week 12. Results： The body weight, kidney weight, serum excretion, serum creatinine, blood urea nitroge were improved by Rg1 ,Rb1 preparation. The expression of VEGF in the Rg1, Rb1 treatment group was depressed greatly in the renal tubular epithelial cell and renal interstitial cell. Conclusion： The overexpression of VEGF in renal of diabetic rats may be one of the mechanisms of diabetic nephropathy, Rg1, Rb1 had certain renoprotective effects.
出处
《中国中医急症》
2008年第9期1263-1264,1267,共3页
Journal of Emergency in Traditional Chinese Medicine
基金
教育部"长江学者和创新团队发展计划"资助项目(No.IRT0413)
国家自然科学基金重点资助项目(No.30130220)
北京市中医管理局"51510"资助项目(No.JJ2007-006)
