摘要
目的:观察灯盏花素对梗阻性肾病大鼠肾组织的保护作用及对血管活性物质的影响。方法:将SD大鼠按照体重不同随机分为假手术组、梗阻性肾病模型组、贝那普利5 mg.kg-1组、灯盏花素100,200 mg.kg-1组,ig给药。假手术组及模型组ig给予0.9%氯化钠溶液,1次/d,连续7 d。给药结束后收集尿液进行N-乙酰-β-D-氨基葡萄糖苷酶(NAG)测定,称重大鼠两侧肾脏,HE及Masson染色观察大鼠肾组织病理改变,免疫组化法检测α-平滑肌肌动蛋白(α-SMA)的表达,放射免疫法、比色法分别检测肾组织中血管紧张素Ⅱ(AngⅡ),血管紧张素转化酶(ACE),一氧化氮(NO)和内皮素-1(ET-1)活性。结果:与假手术组比较,模型组大鼠尿液中NAG酶活性及左侧肾脏质量明显增加(P<0.01),肾间质可见大量炎性浸润及充血,纤维化明显,肾小管明显增宽,ET-1,ACE及AngⅡ升高而NO含量降低(P<0.01)。与模型组比较,贝那普利组及两个灯盏花素实验组尿液中NAG酶活性及左侧肾脏质量减轻(P<0.05,P<0.01),肾组织改善明显。与模型组比较,两个灯盏花素实验组大鼠肾组织中ET-1,ACE及AngⅡ含量降低而NO升高(P<0.05,P<0.01)。结论:灯盏花素可能通过影响肾组织中血管活性物质,下调ET-1,ACE及AngⅡ,上调NO等作用减轻梗阻性肾病。
Objective： To observe the effect of breviscapine on renal tissue vasoactive substances and to analyze the protective mechanism of breviscapine on renal tissue in obstructive nephropathy rat. Method： According to the weight the SD rats were randomly divided into 5 groups, sham operation group（SOG）, obstructive nephropathy model group（MG）, benazepril group（BG）, 100 mgkg-1 breviscapine group（100 mg-BVG） and 200 mgkg-1 breviscapine group（200 mg-BVG）. BG was given benazepril 5 mgkg-1 by gavage （ig）, 100 mg-BVG and 200-mgBVG were given breviscapine 100 mgkg-1 and 200 mgkg-1 gavage, SOG and ONMG were treated with 0.9% sodium chloride solution by gavage, 1/day. The administration lasted 7 days. After administration the rat kidneys were weighed and NAG-U were measured, left renal histopathology were observed by HE and Masson staining; the expression of α-SMA was detected by immunohistochemistry; angiotensin Ⅱ（Ang Ⅱ）, angiotensin converting enzyme （ACE）, nitric oxide （NO） and endothelin （ET-1） content change were measured by radioimmunoassay and colorimetric determination. Result： Compared with the SOG, ONMG rats NAG-U and left renal weight were significantly increased（P〈0.01）, and a large amount of inflammatory cell infiltration and hyperaemia had showed, ET-1, ACE and Ang Ⅱ were obviously increased but the content of NO were significantly decreased（P〈0.01）. Compared with the ONMG, in the BG, 100 mg-BVG and 200 mg-BVG NAG-U and the left renal weight were reduced （P〈0.05,P〈0.01）, inflammatory cell infiltration and hyperaemia were also reduced. Compared with the ONMG, the two BVGs ET-1, ACE and Ang Ⅱcontent were decreased but NO were increased（P〈0.05,P〈0.01）. Conclusion： Breviscapine probably alleviate obstructive nephyropathy by affecting the vasoactive substances, downregulation of ET-1, ACE and Ang Ⅱcontent, the upregulation of NO.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第10期221-225,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
2011年漯河市科技发展计划项目(2011-206-07)
漯河医专2011年度科研课题(2011S04)
关键词
灯盏花素
梗阻性肾病
血管活性物质
breviscapine
obstructive nephropathy
vasoactive substances
