摘要
用反相高效液相色谱法追踪分析不同时间内羊血清中药物浓度的变化，以供药物代谢动力学研究。药物为丙硫咪唑砜和丙硫咪唑亚砜（简称砜和亚砜），实验条件为ＯＤＳ柱，流动相甲醇：水＝７：３（ｖ／ｖ），紫外检测波长为２９０ｎｍ。在３ｈ左右测定浓度达峰值，其最高浓度分别为４．０１μｇ／ｍＬ和１１．４７μｇ／ｍＬ，五天左右下降为２．７３μｇ／ｍＬ和１．３２μｇ／ｍＬ附近。最小检出量为０．４ｎｇ／ｍＬ及０．７ｎｇ／ｍＬ。线性关系良好。丙硫咪唑砜和亚砜的回归相关系数分别为０．９９９４和０．９９９１。采用内标法测定回收率，平均为１００±１２％。
The amounts of sulphone and sulphoxide which are metabolites of albendozle in sheep serum, are quantitatively determined on ODS column by reversed-phase High performance liquid chromatography as the analytical time changes. Mobile phase is a solution of methane:water=0.7; UV detection value is 280 nm.The results show:Firstly,the linear relatious of sulphone is 0.9994; the correlation coefficient of sulphoxide is 0.9991. Secondly, the recovery of sulphone and sulphoxide are determined by internal standard method. The average recovery is 100+12% .Thirdly, the concentration of the supersession of albendozle gets high point after tfree hours metabolism. As the metabolic time increases, the concentration of the supersession of albendozle decreases. The sensitivity limit of sulphone and sulphoxide are 0.4μg/mL and 0 7μg/mL recpectively。
出处
《分析测试技术与仪器》
CAS
1997年第4期250-253,共4页
Analysis and Testing Technology and Instruments
关键词
丙硫咪唑砜
丙硫咪唑亚砜
驱虫药
HPLC
羊血清
high-performance liquid chromatography albendozle sulphone albendozle sulhoxide
