摘要
目的为了建立快速检测长爪沙鼠群体遗传多样性的方法及获得Z:ZCLA长爪沙鼠封闭群现用微卫星位点的结构。方法利用17个微卫星位点(9个来自长爪沙鼠,8个来自大小鼠)进行了PCR反应体系及反应条件的优化,组合了6组双重PCR及两个复合式点样,用上述8个组合对普通级Z:ZCLA长爪沙鼠封闭群43、444、5三个世代核心群各100只种鼠进行遗传检测。结果三个世代的300只种鼠的检测结果表明,9个长爪沙鼠位点均为微卫星,其中7个位点为完全型的微卫星,1个为复合型,1个为不完全型,多态性主要表现在核心序列的重复;来自大小鼠的8个微卫星位点,有7个在Z:ZCLA长爪沙鼠核心群中得到有效扩增,只有3个位点在三个世代中均有出现,对测序结果分析后发现,其核心序列均为小卫星。结论来自长爪沙鼠的位点,无论结构还是遗传方式均符合微卫星遗传标记的特点,可用作检测长爪沙鼠的群体遗传多样性。
Objective In order to develop a rapid technique to detect the genetic diversity of Mongolian gerbil and survey the microstatellite structure in the Z：ZCLA closed gerbil population. Methods Duplex-PCR technique was employed to survey the genetic diversity of rat- and mouse-originated microsatellite loci in gerbil in comparison with the gerbil-originated loci among 43-45 genarations of Z ： ZCLA closed population. Results The microsatellite structure of these 9 loci were resolved and three types could be discriminated out. They were compound microsatellite type （1 locus）, imperfect microsatellite type （1 locus）, and perfect microsatellite type （7 loci）, respectively. The polymorphism of core sequence was mainly originated from the number of the core repeat units. Seven of eight rat- and mouse-originated microsatellite loci could give PCR products in laboratory gerbils. Three loci of them presented in each generation tested. The results of sequence analysis showed that the amplification products of these three loci appeared minisatellite patterns. Conclusion The gerbil-originated loci may serve as marker to assess the genetic diversity of laboratory gerbils.
出处
《中国实验动物学报》
CAS
CSCD
2008年第4期282-288,共7页
Acta Laboratorium Animalis Scientia Sinica
基金
浙江省科技厅重大项目(编号:2004F11014)
关键词
长爪沙鼠
微卫星位点
微卫星结构
Mongolian gerbil
Microsatellite markers
Microsatellite structure
