摘要
目的通过检测急性冠状动脉综合征患者(ACS)外周血中调节性T细胞(Treg细胞)的数量变化,了解Treg细胞与ACS发生发展的关系及意义。方法入选40例急性心肌梗死患者(心梗组)、40例不稳定型心绞痛患者(心绞痛组)和40名健康人(对照组)。所有入选受试对象外周血血液样本均采用流式细胞术检测CD4+CD25+CD127-的结果来定义各组外周血中Treg细胞表达的百分比,比较各组的Treg细胞表达百分比,同时比较心梗后12、48、72h Treg细胞表达百分比变化。常规检测血常规、血脂。结果外周血中Treg细胞表达的百分比[心绞痛组(3.20%±0.92%)比对照组(3.76%±1.18%)]有所下降,差异有统计学意义(P<0.05),心梗后12h(2.12%±0.89%)患者外周血中Treg细胞表达的百分比也明显下降(P<0.001);心梗后12 h(2.12%±0.89%)、48 h(3.0%±0.89%)、72 h(3.57%±0.92%)的Treg细胞百分比逐渐递增。心梗后各时间点两两比较,差异均存在统计学意义(P<0.05)。心梗后72h Treg细胞百分比与对照组相比差异无统计学意义。心梗组常规检测血常规中白细胞[(10.34±2.48)×109/L]、中性粒细胞(0.80±0.06)较正常值显著异常升高,且中性粒细胞与Treg细胞百分比呈负性相关(相关系数r为-0.517,P<0.05)。结论急性冠脉综合征患者Treg细胞数量减少,且与炎症反应呈负性相关,故可推测Treg细胞是ACS患者免疫稳态失衡和炎症反应的标志物之一。
Objective To investigate the abnormality of CD4 ＋ CD25 ＋ regulatory T cell in peripheral blood of patients with acute coronary syndrome（ACS） and to identify its relationship with arteriosclerosis. Methods Flow cytometry was used to detect the frequencies of CD4 ＋ CD25 ＋ regulatory T cells in peripheral blood from 40 patients with acute myocardial infarction （AMI）, 40 patients with unstable angina （UA）, and 40 normal individuals. All patients had been ruled out infection, disease of immune system, malignant tumor and so on. The frequencies of regulatory T cells in all patients were detected. Levels of CD4 ＋ CD25 ＋ CD127 - in circulation were measured to determine the number of Treg cells by flow cytometry. Meanwhile, the frequencies of CD4 ＋ CD25 ＋ regulatory T cells in peripheral blood were detected in AMI patients after 12 h, 48 h and 72 h. Results Compared to controls, the frequencies of CD4 ＋ CD25 ＋ regulatory T cells in peripheral blood of AMI and UA group were significantly decreased （ all P 〈 0. 05 ）. The frequencies of CD4 ＋ CD25 ＋ regulatory T cells in peripheral blood 12 h, 48 h and 72 h after AMI were increased progressively. Compared to 12h, the frequencies of Treg cells in peripheral blood 48 h and 72 h after AMI were significantly raised （ P 〈 0. 05 ）. In contrast ,the levels of WBC [ （ 10. 34 ± 2.48 ） × 10^9/L ] and percent neutrophils （ 0. 80 ± 0. 06） in AMI group were markedly higher than those in the UA group and controls （ all P 〈 0. 05 ）. There was a negative correlation between the frequencies of CD4 ＋ CD25 ＋ regulatory T cells and neutrophil in AMI group （ r = - 0. 517, P 〈 0.05 ）. Conclusion In ACS patients, the decrease in frequencies of Treg may break the balance of peripheral immunotolerance and participate in pathological process of inflammatory response inducing the development of ACS.
出处
《中国心血管杂志》
2008年第4期258-261,共4页
Chinese Journal of Cardiovascular Medicine
关键词
冠状动脉疾病
T淋巴细胞
调节性
全身炎症反应综合征
Coronary artery disease
T-lymphocyte, regulatory
Systemic inflammatory response syndrome
