摘要
目的研究乳腺叶状囊肉瘤增殖细胞核抗原（ＰＣＮＡ）的表达及其临床病理意义。方法采用免疫组化ＳＰ法对１００例乳腺叶状囊肉瘤及３９例乳腺腺纤维瘤进行检测。结果叶状囊肉瘤中间质性瘤细胞呈ＰＣＮＡ阳性着色者８６例（８６％），不同组织学级别者ＰＣＮＡ指数之间有显著差异（Ｆ＝８５．３３，Ｐ＜０．０１），分化越差，ＰＣＮＡ平均指数越高，ＰＣＮＡ指数与组织学分级密切相关（ｒ′ｓ＝０．７７）。Ⅰ级叶状囊肉瘤与乳腺腺纤维瘤间质性瘤细胞丰富组的ＰＣＮＡ指数也有显著差异（ｔ＝３．４２，Ｐ＜０．０１）。ＰＣＮＡ指数与核分裂相计数间相关关系并不密切（ｒ＝０．３９）。结论用ＰＣＮＡ来反映乳腺叶状囊肉瘤增殖活性，辅助进行组织学分级，鉴别良恶交界性病变及估测预后，都具有一定的实用价值。
Objective To investigate the expression of proliferating cell nuclear antigen (PCNA) in breast phylloides cystosarcoma and its clinicopathological signification. Methods Immunohistocemhistry (SP method) with monoclonal antibodies PC10 against PCNA was performed in 100 cases of phylloides cystosarcoma and 39 cases of adenofibroma. Results The positive rate of PCNA in phylloides cystosarcoma was 86%. The average PCNA index (PI) of phylloides cystosarcoma was significantly different among every histologic grading (F=85.33,P<0.01). The degree of differentiation was lower,the PI was higher. The PI was closely correlated with histologic grading (r′s=0.77). The PI in grade I phylloides cystosarcoma was higher than that in the group of adenofibroma with abundant mesenchymal cells (t=3.42, P<0. 01). There was only a low correlation between PI and mitotic figures in phylloides cystosarcoma (r=0.39). Conclusions These findings suggest that the detection of PCNA has considerable practical value in reflecting the proliferation of activity of phylloides cystosarcoma, assisting the pathologists to make histologic grading; distinguishing the malignancy from benign ones (differential diagnosis) and evaluating the prognosis.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1997年第6期337-339,共3页
Chinese Journal of Pathology
关键词
乳房肿瘤
增殖细胞核抗原
免疫组织化学
表达
Breast neoplasms Proliferating cell nuclear antigen Adenofibroma Immunohistochemistry
