摘要
目的探讨核黄素光化学法对红细胞悬液中病毒灭活效果。方法以人巨细胞病毒(HCMV)作为指示病毒,在红细胞悬液中分别加入不同浓度的核黄素,结合照度为40000 Lux的可见光(λ(600nm),处理后加入单层人胚成纤维细胞(HF)中培养,观察细胞病变效应并测定病毒滴度,用PCR检测病毒核酸的变化。结果红细胞悬液中的核黄素浓度为50、100、150、200μmol/L时,结合照度为40000 Lux的可见光分别照射40、30、20、20min时,可将滴度为7.65log TCID50的HCMV灭活至<0.50log TCID50;PCR法未能检测出病毒核酸。结论核黄素光化学法能有效灭活红细胞悬液中的HCMV。
Objective To develop a photochemical method that can inactive human cytomegalovirus in red blood cell. Methods Human cytomegalovirus （HCMV） was as model virus. The contaminated red blood cell suspension was treated with different concentration riboflavin and visible light. TCID50 was measured to assess virus inactivation efficacy. A segment of nucleic acid of HCMV was amplified with PCR. Results When concentration of riboflavin in red blood cell suspension was 50, 100, 150 and 200 μmol/L, and illumination of visible light （40 000 Lux） for 40,30,20, and 20 min reduced the titter of HCMV in the suspension from 7.65 log TCID50 to 〈0. 50 log TCID50. Conclusion Riboflavin photochemical can effectively inactivate HCMV in red blood cell suspension.
出处
《安徽医科大学学报》
CAS
北大核心
2008年第4期402-405,共4页
Acta Universitatis Medicinalis Anhui
关键词
病毒灭活
核黄素/药理学
巨细胞病毒
红细胞输注
virus inactivation
riboflavin/pharmacology
cytomegalovirus
erythrocyte transfusion
