摘要
目的:探讨维生素B_2光化学病原体灭活技术(PRT)处理的单采血小板对白细胞和血小板源细胞因子释放的影响。方法:随机选取捐献血小板的献血员20人,各取60 ml去白细胞单采血小板,平均分成2份:一份不做处理作为对照组,另一份采用维生素B_2联合紫外光照射的光化学法处理作为实验组。在血站标准条件下保存7d,分别于1、3、5和7 d取样,分析血小板计数(PC)、血小板分布宽度(PDW)、平均血小板体积(MPV);应用ELISA方法检测白细胞源细胞因子(IL-1β、IL-2、IL-6、IL-8、TNF-α和IFN-γ)和血小板源细胞因子(CCL3、CCL5、TGF-β-1和PF4)含量以及可溶性P-selectin含量;流式细胞术检测血小板膜磷脂酰丝氨酸(PS)表达水平。结果:实验组与对照组相比,PC、PDW和MPV没有统计学差异。随着血小板贮存期的延长,血小板源性细胞因子含量逐渐增加,5-7 d时达到一个新的高度平台,且实验组中血小板源性细胞因子的含量显著高于对照组(P<0.05)。在血小板贮存的3、5和7 d时,实验组中PS表达阳性率显著高于对照组(P=0.0052,P=0.010,P=0.011)。在5和7 d时,实验组中P-selectin含量显著高于对照组(P=0.0138,P=0.0036)。白细胞源性细胞因子含量在实验组和对照组中相比较差异无统计学意义。结论:经PRT处理的去白细胞血小板中,细胞因子的释放主要来源于血小板,在贮存5和7 d时,血小板源性细胞因子的积累达到一个新的平台,其原因最可能是血小板的活化和凋亡。
Objective： To investigate whether vitamin B2 photochemical pathogen reduction technology（ PRT treatment may lead to increase white cell-and platelet-derived cytokines release from platelets during storage. Methods：Sixty milliliters of leukodepleted apheresis platelets were collected from 20 healthy donors,then were divided into 2parts： one part（ 30 ml） remained untreated to serve as control,while the other part was treated with vitamin B2-UVB photo-chemical technology as experimental group. During 7 d of storage under standard blood bank conditions,platelet coun-ting（ PC）,platelet distribution width（ PDW）,mean platelet volume（ MPV）,white cell-derived cytokines（ IL-1β,IL-2,IL-6,IL-8,TNF-α and IFN-γ） and platelet-derived cytokines（ CCL3,CCL5,TGF-β-1 and PF4）,Pselectin and phosphatidyl serine（ PS） were analyzed on day 1,3,5 and 7 of storage,respectively. Results： No significant differences were observed on PC,PDW and MPV between the experimental and control groups,respectively. The higher levels of platelet-derived cytokines were detected and reached a plateau after 5-7 days of storage,and the cytokines showed significant increase in experimental group compared with the control group. PS expression increased significantly in experimental group as compared with control group on day 3,5 and 7 of storage,respectively. The accumulation of P-selectin was significant higher in experimental group than that in control group on day 5 and 7 of storage（ P〈0. 05）. The white cell-derived cytokines were not elevated by PRT treatment during 7 days of storage. Conclusion： The PRT-treated platelets are the main source of released cytokines during storage of PRT treatment. The levels of platelet-derived cytokines reach a plateau after 5-7 days of storage,most likely due to accelerated platelet activation and apoptosis.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2017年第4期1212-1217,共6页
Journal of Experimental Hematology
基金
山东省优秀中青年科学家科研奖励基金(BS2011SW044)
山东省医药卫生科技发展计划项目(2013WS0170)
