摘要
目的:观察三七总苷对应激大鼠血清及胃黏膜氧自由基的影响。方法:采用经典水浸-束缚应激模型,三七总苷组(PNS 66mg/kg)预防性灌胃给药3天,检测大鼠血清、胃黏膜SOD、MDA、NO含量。结果:水浸-束缚应激6 h后模型组胃黏膜NO含量比正常对照组降低,MDA含量增加,SOD活性下降;PNS治疗组,NO含量略升高,MDA含量下降,SOD活性增高;模型组血清NO含量比正常对照组降低,MDA含量增加,SOD活性下降;PNS治疗组血清NO含量升高,但MDA含量和SOD活性与模型组比较差异无统计学意义。结论:水浸-束缚应激可造成大鼠氧自由基损伤,氧自由基损伤主要发生在胃黏膜。PNS能够清除氧自由基,抑制过氧化物产生,对应激大鼠胃黏膜有一定的保护作用。
Objective ： To observe the effect of Total Saponins of Panax notognseng （PNS） on oxygen free radicals （OFR） levels in serum and gastric mucosa of stress rats. Methods： Water-immersion restraint stress （WRS）was used to induce rat models of stress. Thirty-two SD rats were randomized into the normal group, model group, PNS group （66mg/kg）, and ranitidine group（12.5mg/kg）. The rats were pretreated with corresponding drugs or the same volume of distilled water. After treatment, superoxide dismustase （ SOD）, malondialdehyde （MDA） and nitric oxide （NO）contents in serum and gastric mucosa of stress rats were detected. Results： Six hours after WRS, the NO content and SOD activity in gastric mucosa were lower, and MDA content was higher in the model group than those in the normal group （ P 〈 0. 05 ）. After treatment with PNS, NO content in gastric mucosa was increased, MDA decreased （P 〈 0.05） and SOD activity increased （P 〈 0.01 ）. In the serum of the model group, NO content and SOD activity were lower, and MDA content was higher than those in the normal group （ P 〈 0. 01 ）. After treatment with PNS, serum NO content was increased （ P 〈0.05）, but there were no obvious changes of MDA and SOD. Conclusion： WRS can induce the injury of oxygen free radicals in rats. PNS has protective effect on gastric mucosa of stress rats by clearing oxygen free radicals and inhibiting the production of peroxides.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2008年第3期41-43,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
广州中医药大学中医药创新基金课题(2006F31)
关键词
三七总苷
应激
氧自由基
NO
SOD
MDA
Total Saponins of Panax notognseng
oxygen free radicals
nitric oxide
superoxide dismustase
malondialdehyde
