摘要
背景:血管脱细胞组织基质作为天然生物支架材料的制备与临床应用具有一定的时差,保存条件对其影响逐渐收到人们重视。目的:拟观察不同液氮保存时间对脱细胞血管基质支架材料组织形态及力学特性的影响。设计、时间及地点:随机分组设计,对比观察,于2007-08/2008-05在青岛市市立医院心脏外科实验室完成。材料:健康新西兰兔10只,体质量550~600g,用于制备脱细胞血管基质。方法:采用胰蛋白酶、低渗溶液、化学除垢剂法处理兔胸主动脉制备脱细胞血管基质。根据液氮保存时间的不同将制取的血管随机分为液氮1月组,液氮2月组和液氮3月组;另设新鲜组:未用液氮保存而作为对照。主要观察指标:苏木精-伊红染色、扫描电镜观察各组脱细胞血管基质形态变化,并作力学测试,包括Lagrange应变、Lagrange应力、伸长比和极限应力。结果:苏木精-伊红染色可见新鲜组血管脱细胞组织基质中胶原纤维和弹性纤维保持原来的形态和结构,呈网状排列,细胞已基本去除。液氮保存组光镜下见与新鲜组无明显差异。扫描电镜见新鲜组血管脱细胞组织基质的纤维结构完整,胶原纤维无断裂,为网状和多孔状,孔径为(100~150)×(10~20)μm。液氮保存组胶原纤维结构完整,呈网状排列,胶原纤维无断裂现象,孔径与新鲜组无明显差异。各组力学指标相比,差异无显著性意义(P>0.05)。结论:不同液氮保存时间对脱细胞血管基质的形态学、组织学以及力学特性方面没有明显影响。
BACKGROUND： As a natural scaffold biomaterial, acellular tissue matrix deserves further experiences for its clinical application, and the effect of preservation condition has attracted more and more attention. OBJECTIVE： To observe the influence of liquid nitrogen preservation time on the morphology and mechanical characteristics of acellular vessel matrix. DESIGN, TIME AND SETTING： A randomized control observation was completed in the Laboratory of Cardiovascular Surgery, Qingdao Municipal Hospital （Qingdao, Shandong, China） between August 2007 and May 2008. MATERIALS： Ten New Zealand rabbits weighing 550-600 g were used to prepare acellular vessel matrix. METHODS： Trypsin, hypertonic solutions and chemical detergent were applied for a multistep process to carry out the preparation of acellular vessel matrix from rabbit thoracic aorta. According to the liquid nitrogen preservation time, the vascular specimens were divided into four groups randomly, namely fresh group, 1 month group, 2 months group and 3 months group. Fresh group was not subjected to liquid nitrogen preservation. MAIN OUTCOME MEASURES： Specimens were detected by hematoxylin-eosin stain and observed by scanning electron microscope for the mechanics test, including Lagrange strain, Lagrange stress, extension ratio and ultimate stress. RESULTS： Hematoxylin-eosin stain results showed that, collagen fiber and elastic fiber in the aceUular vessel matrix of fresh group retained the previous morphology and mesh structure, the cells were almost removed. Under scanning electron microscope, the collagen fiber of both groups was intact without fragmentation, showing a mesh or porous structure. The porosity was also identical between the two groups, （100-150）^（10-20） 1~ m. There were no obvious differences between liquid nitrogen groups and fresh group in the morphology and mechanical characteristics （P 〉 0.05）. CONCLUSION： There are no obvious influence in the morphology, histology and mechanical characteristics of acellular vessel matrix by the different liquid nitrogen preservation time.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第27期5235-5238,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
青岛市科技局基金项目(06-2-2-6-nsh)~~
