摘要
目的：对自主研制脉动生物反应器加以改进，使其脉动流场能够体外模拟高流量高压力的在体瓣膜环境，并对整体构建的组织工程心脏瓣膜（TEHV）进行流场内培养的初步研究。方法：采用韩国产双囊搏动泵（T-PLS）作为新脉动流场的动力装置，并设计了新的气液交换通路以减少污染。以脱细胞猪主动脉瓣为支架，人的骨髓间质干细胞（BMSCs）为种子细胞整体构建TEHV。静态培养4d后，置于生物反应器内分别在小流量（0～600ml／min）和大流量（0～4800ml／min）脉动流场培养7d，观察细胞残留情况。结果：流场改进后流量范围从0～1200ml／min提高到0～6000ml／min，系统内压力范围从0～40mmHg（1mmHg=0．133kPa）提高到0～180mmHg。TEHV在低流量脉动流场内有部分细胞存留（26．3％），而在大流量流场中细胞几无存留。结论：新的生物反应器能够基本模拟在体瓣膜的流体动力学环境，能够应用于TEHV的体外培育研究，但目前构建的TEHV尚不能耐受大流量流体的冲击。
Objective： To modify the pulsatile bioreactor we constructed previously for simulating the high-flow, highpressure hemodynamics of heart valve in vivo, and to experimentally cultivate the tissue-engineered heart valves （TEHV） in the modified bioreactor. Methods： T-PLS system （NewheartBio Co. ,Ltd Korea） was used to generate pulsatile flow in the modified bioreactor and we designed a new air-exchange pathway to avoid contamination. The TEHV were made by seeding human bone mesenchymal stem cells （BMSCs） on deellularized porcine heart valve. After cultured under static condition for 4 d, the TEHVs were moved to the modified bioreactor and exposed to low-flow （0-600 ml/min） or high-flow（0-4 800 ml/min） pulsatile hydrodynamics for 7d, then the cells on TEHVs were evaluated. Results： After modification, the flow range expanded from （0- 1 200） ml/min to （0-6 000） ml/min and the pressure range expanded from （0-40） mmHg to （0-180） mmHg. In culture experiments, 26.3 % of the seeded cells remained under low-flow environment and cells were completely lost under the high-flow dynamics. Conclusion： The modified bioreactor can basically simulate the dynamics of heart valve in vivo and can be used in TEHV cultivation research. However, the current TEHV can not tolerate the high-flow pulsatile hydrodynamics.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2007年第1期13-15,共3页
Academic Journal of Second Military Medical University
基金
全军医药科研"十五"规划重点课题(01Z060)~~
关键词
组织工程
心脏瓣膜
人工
生物反应器
tissue engineering
heart valve prosthesis
bioreactors
