摘要
目的建立抗菌消炎片中绿原酸含量测定方法。方法采用HPLC法,色谱柱：日本岛津VP-ODS柱（150 mm×4.6 mm,5μm）;流动相：乙腈-0.4%磷酸溶液（13∶87）;检测波长：327 nm;流速：1 ml·min^-1;柱温：30℃。结果绿原酸在0.098～0.590μg范围内呈良好线性（r=0.9999）,平均回收率为100.6%（RSD为1.1%,n=6）。结论本方法简便,准确,可作为该制剂的质控标准。
Aim To establish a method for determination of chlorogenic acid in kangjun xiaoyan tablet.Method Shim-Pack VP-ODS column was used.The mobile phase was acetonitrile-0.4%phosphric acid solution（13∶87）.The detection wavelength was 327nm.The flow rate was 1 ml·min^-1.The column temperature was at 30℃.Results Ephedrine hydrochloride showed a good linear relationship at the range of 0.098～0.590 μg （r=0.999 9）.The average recovery and the relative standard deviation was 100.6% and 1.1%（n=6）.Conclusions The method was simple and accurate.This method is suitable for the quality control of this preparation.
出处
《安徽医药》
CAS
2008年第9期797-798,共2页
Anhui Medical and Pharmaceutical Journal
关键词
HPLC
抗菌消炎片
绿原酸
HPLC
kangjun xiaoyan tablet
chlorogenic acid
