摘要
采用RT-PCR方法从番茄果实cDNA中成功克隆了番茄LeEIL1基因,并测序验证序列正确;经数据库检索等生物信息学分析方法,对番茄LeEIL1蛋白质与拟南芥、烟草、水稻、香石竹等的EIN3/EILs蛋白质序列进行了同源性分析,初步确定了番茄LeEIL1上的DNA结合功能域及其结合激活位点。并构建了LeEIL1的酵母表达工程菌pPIC9k-EIL1/KM71,为该基因的功能研究奠定了基础。
Tomato LeEIL1 gene was cloned by RT-PCR from tomato fi-uit cDNA. After homology analysis of tomato LeEIL1,Arabidopsis thaliana EIN3,Nicotiana tabacum EIL,Oryza sativa EIL1,Dianthus caryophyllus EIL1,Musa acuminate EIL2 and Vigna radiate EIL1 proteins in some protein model databases,the DNA binding domain and binding activity site of tomato LeEIL1 protein were probably identified. Then,LeEIL1 was cloned into pPIC9k expression vector, the yeast expression strains pPIC9k-EIL1/KM71 were successfully selected.
出处
《生物技术通报》
CAS
CSCD
2008年第4期114-117,共4页
Biotechnology Bulletin
基金
项目支持国家自然科学基金(30371006)
中法先进研究项目(PRABT-04-01)
