摘要
以经超临界CO2萃取除去大部分蚕蛹油的脱脂蚕蛹蛋白质为原料,研究不同酶种类制备蚕蛹活性肽的酶解效果,选用碱性内切蛋白酶水解蚕蛹蛋白质制备活性肽,研究水解温度、酶用量([E]/[S])、底物浓度([S])和pH值等因素变化时水解度DH随时间的变化规律,结果表明,以DH为考察指标得到该碱性内切蛋白酶水解蚕蛹蛋白质的适宜条件为:水解时间300min,酶用量[E]/[S]为0.3%,[S]为8%,pH值9.5,水解温度50℃,此时DH为22.66%,酶解物主要由小肽分子组成。
After silkworm pupa was degreased by supercritical extraction of carbon dioxide and ethanol, Bioactive peptides with alkali protease hydrolyzing were prepared from defatted silkworm pupa protein. The relations between degree of hydrolysis （DH） of hydrolysate and its hydrolysis time were studied while the technical conditions were changed such as hydrolysis time, hydrolysis temperature, the ratio of enzyme to substrate （[E]/[S]）, the concentration of substrate （[S]） and pH. The result showed that the optimal technical condition hydrolyzing defatted silkworm pupa protein was hydrolysis time 300 min, hydrolysis temperature 50 ℃, [E]/[S]0.3%, [S]8% and pH9.5, then the DH was 22.66%, and the hydrolysates were mostly composed of oligopeptides in the products.
出处
《食品科技》
CAS
北大核心
2008年第8期177-180,共4页
Food Science and Technology
基金
广西自然科学基金资助项目(桂科自0728237)
广西教育厅科研项目(200607MS031)
广西大学基金资助项目(X071029)
关键词
酶解
蚕蛹蛋白
工艺条件
enzymatic
silkworm pupa protein
bioactive peptides
