摘要
采用重量法对可可茶（ＣａｍｅｌｉａｐｔｉｌｏｐｈｙｌａＣｈａｎｇ）嫩芽的嘌呤生物碱进行了分离和测定，用硅胶薄层层析ＴＬＣ，对所得到产物与咖啡碱、可可碱和茶叶碱的标准品比较，表明重量法分离得到的是咖啡碱和可可碱，且纯度甚高．又用核磁共振１ＨＮＭＲ对所得的产品进行了化学结构的测定，也证明它们分别与标准品咖啡碱和可可碱的完全一致．由此再一次证实纯系可可茶嘌呤生物碱以可可碱为主．研究还以从原生地移植的２２株可可茶进行了种内个体嘌呤生物碱表现的研究，结果表明２２株茶树中有１８株嘌呤生物碱以可可碱为主，不含咖啡碱，另外４株则以咖啡碱为主，也含少量的可可碱．
Purine alkaloids from flushed shoots of Camellia ptilophylla Chang and other tea trees were isolated and analyzed by exhaustive extraction.The products were detected by TLC and 1H NMR and are identical to the standards of caffeine and theobromine espectively. It shows that C. ptilophylla is different from cutivate tea trees with containing mainly coffeine. Twenty two trees were surveyed by TLC and HPLC, eighteen of them mainly contained theobromine ranging from 4 97% to 6 84% and without caffeine; and four of them not only contained predominant caffeine but also theobromine. It is necessary to continue studying the stability that C ptilophylla contains predominant theobromine from heredity.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
1997年第6期30-33,共4页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家自然科学基金
关键词
可可茶
嘌呤生物碱
分离
Camellia ptilophylla, purine alkaloids, isolation, analysis
